小檗碱对2型糖尿病ICR小鼠模型的治疗作用  

Therapeutic Effect of Berberine Recipe on ICR Mouse Model of Type 2 Diabetes

作　　者：张玲玲[1] 黄澜[1] 徐艳峰[1] 李加美[1] 肖冲[1] 刘颖[1] 秦川[1] 

机构地区：[1]中国医学科学院实验动物研究所,北京协和医学院比较医学中心,北京100021

出　　处：《中国比较医学杂志》2010年第1期23-27,I0006,I0007,共7页Chinese Journal of Comparative Medicine

摘　　要：目的比较小檗碱及小檗碱与添加剂对2型糖尿病ICR小鼠模型的降糖、降脂效果。方法100只三周龄雄性ICR小鼠随机分为正常组、模型组、小檗碱组、小檗碱加低剂量添加剂组、小檗碱加高剂量添加剂组及二甲双胍组,以高糖高脂加尿链佐菌素诱导小鼠2型糖尿病模型,并分别以小檗碱、小檗碱加低剂量添加剂、小檗碱加高剂量每天灌胃干预治疗6周。检测血糖、血清血脂、肾功指标的变化情况;给药前后做糖耐量胰岛素耐量实验。每周量小鼠体重,动态观察小鼠体重变化。代谢笼中测量小鼠饮食水量变化。结果模型组血糖较普食组显著上升(P<0.01),且维持较好;血脂指标CHO、HDL-c、LDL-c均较普食组升高(P<0.05);其他各检测生化指标差异不显著。小檗碱组空腹血糖较模型组总体下降趋势明显且维持较好(P<0.01);血脂较模型组有降低;糖耐量胰岛素耐量较模型组有改善。二甲双胍组血糖较模型组有下降趋势,血脂较模型组无显著差别。小檗碱加低剂量添加剂组较模型组血糖血脂无显著差别。小檗碱加高剂量添加剂组血糖较模型有下降趋势,血脂较模型组有下降趋势,其他各项生化指标均无差别。结论小檗碱降糖效果优于二甲双胍,小檗碱加低剂量添加剂,小檗碱加高添加剂。Objective To compare the effect of berberine and additive on serum glucose and lipid level.MethodsType 2 diabetic model was induced by both high lipid food and low dose of streptozotocin（STZ） in mice.The number of 100 three month＇s mice were randomly divided into normal group,type 2 diabetic model group,berberine treated group,berberine and low dose additive treated group,berberine and high dose additive treated group and metformin treated group.Every group were administered through intragastric administration of berberine,berberine and low dose additive,berberine and high dose additive in six weeks.The capability of glucose and insulin tolerance was measure.The blood glucose,the serum lipids and the function of kidney were observed.The weight of mice was monitored every week.Food and drink of the mice were also observed in the metabolic cages.Results The blood glucose level of type 2 diabetic model was obviously higher than that of normal group（P〈0.01） and steady.The level of CHO,HDL-c and LDL-c in the blood lipids were increased,other indexes were not statistical significance.The glucose level of Metformin treated group was decreased,but the blood lipid level was no significant difference.The glucose level and blood lipid level of berberine and low dose of additive treated group were no obvious difference.The glucose level of berberine and high dose additive treated group were decreased,but the blood lipid level was no significant difference.Other biochemical indexes showed no difference.Conclusions／ The medical activities of berberine are better than metformin,berberine and low dose additive,berberine and high dose additive.

关 键 词：小檗碱 2型糖尿病模型 

分 类 号：R-33[医药卫生]