PTEN抑癌基因对人黏液表皮样癌细胞系增殖和细胞周期的影响  

Effects of PTEN tumor suppressor gene on cell proliferation and cell cycle in human mucoepidermoid carcinoma cell line

作　　者：刘斌[1] 吴军正[1] 徐小方[1] 王哲[1] 谭新颖[1] 

机构地区：[1]第四军医大学口腔医学院生物学教研室,陕西西安710032

出　　处：《中国口腔颌面外科杂志》2010年第1期50-54,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(30672343)~~

摘　　要：目的:探讨外源性PTEN抑癌基因对唾液腺黏液表皮样癌细胞系增殖抑制的机制。方法:将含野生型PTEN抑癌基因cDNA重组反转录病毒表达质粒导入高转移性唾液腺黏液表皮样癌细胞系M3SP2,转染空载体细胞为对照。MTT比色法测定细胞增殖,流式细胞术测定细胞周期,免疫组化染色检测PCNA、EGFR、CDK4、P16INK4a和P57Kip2蛋白表达。采用SPSS11.0软件包进行统计学分析。结果:与对照细胞比较,PTEN基因转染癌细胞M3SP2-PTEN对表皮生长因子(EGF)介导的细胞增殖具有显著的抑制作用,癌细胞阻滞在G0/G1期,PCNA、EGFR、CDK4以及C-myc蛋白表达减弱,而P16INK4a和P57Kip2蛋白表达增强(P<0.05)。结论:外源性PTEN基因具有抑制唾液腺黏液表样癌皮细胞系EGF介导的增殖作用,其作用机制与细胞周期进程受阻以及细胞周期调控分子有关。PURPOSE： Phosphatase and tensin homologue deleted on chromosome 10 （ PTEN ） is a tumor suppressor gene which might play an important role in cell proliferation, cell cycle and apoptosis of cancer cells. In the present study, we investigated the effects of exogenous wild-type PTEN gene on proliferation of the mncoepidermoid carcinoma cell line from salivary gland and related mechanisms. METHODS： The recombinant retrovirus expression vector containing human wild-type PTEN gene packaged with lipofectin were introduced into mucoepidermoid carcinoma cell line M3SP2 and parental M3SP2 cells were transfected with empty vector served as controls. The effects of growth factors on the cell viability were analyzed by MTT assay. Cell cycle was analyzed using flow cytometry. The expression of PCNA,EGFR, CDK4,P16INK4a and P57Kip2 protein in cells was analyzed by immunohistochemical methods.SPSS 11.0 software package was used for statistical analysis. RESULTS： The results showed that EGF-mediated cell proliferation in cancer cells transfected＇with wild-type PTEN gene was significantly suppressed, the accumulation of cells in the G0/G1 phase increased, the expression of PCNA,EGFR,CDK4 and C-myc decreased and the levels of P16INK4a and P57Kip2 increased, compared with control cells （P〈0.05）. CONCLUSION： This study suggests that the exogenous wild-type PTEN gene can significantly inhibit EGF-mediated cell growth stimulation in mucoepidermoid carcinoma cells and its mechanism is associated with G0/G1 arrest and related cell cycle regulators. Supported by National Natural Science Foundation of China （Grant No.30672343）.

关 键 词：PTEN抑癌基因 黏液表皮样癌细胞 细胞周期 细胞增殖 

分 类 号：R739.8[医药卫生—肿瘤]