光化学法诱导兔视网膜动脉阻塞模型的建立  

Rabbit model of retinal artery occlusion established by photochemistry therapy

作　　者：刘岩[1] 张瑞帆[2] 翁欢[1] 李秋华[1] 

机构地区：[1]复旦大学附属华山医院眼科,上海200040 [2]四川省人民医院眼科,成都610072

出　　处：《复旦学报（医学版）》2010年第1期106-109,共4页Fudan University Journal of Medical Sciences

基　　金：上海市经委课题(沪创新J-59-3)

摘　　要：目的探讨应用倍频532 nm激光通过光化学法建立视网膜动脉阻塞(retinal artery occlusion,RAO)动物模型的可行性。方法健康成年新西兰白兔10只,自耳缘静脉注入5%孟加拉红,应用倍频532 nm激光通过光化学法制备RAO模型,并于1 h、24 h、3 d行直接眼底镜、眼底照相和眼底荧光血管造影(fundus fluorescein angiography,FFA)检查;3 d后将实验兔以空气栓塞法处死,摘除眼球行组织学检查。结果9只实验眼经直接眼底镜观察和FFA检查证实建立RAO,眼球组织切片光镜下检查可见视网膜动脉内血栓,血管壁基本完整。结论应用用倍频532 nm激光通过光化学法能成功建立兔RAO模型,且组织学检查证实动脉血栓形成同时并无明显的血管壁损伤;此方法成功率高、安全性好、重复性好,可作为探索RAO治疗方法的试验动物模型。Objective To establish an experimental retinal artery occlusion（RAO） model by means of photochemistry therapy with 532 nm frequency-doubled laser,and to evaluate the feasibility of this method. Methods Ten eyes from ten healthy adult New Zealand rabbits were intravenously injected 5% rose bengal solution,then their monocular retinal artery was irradiated by 532 nm frequency-doubled laser.Both eyes of these rabbits were examined by direct ophthalmoscope and fundus photographic camera on the 1st hour,24^th hour and 3rd day.Meanwhile,the experimental eyes were examined by fundus fluorescein angiography（FFA）.These rabbits were killed on the 3rd day,experimental eyes were enucleated,the retina was observed under light microscope. Results There were 9 eyes approved having RAO by direct ophthalmoscope and FFA.Thrombi in retinal arterials could be found by light microscope in all these 9 experimental eyes,and the walls of blood vessel were intact without any obvious necrosis or attenuation. Conclusions RAO animal model can be induced by photochemistry therapy with 532 nm frequency-doubled laser.There are thrombi in the retinal artery without trauma of the artery wall.Photochemistry therapy is an efficient,secure and reproducible method to induce animal model of RAO.

关 键 词：光化学法 视网膜动脉阻塞 动物模型 

分 类 号：R774[医药卫生—眼科]