N-乙酰半胱氨酸保护大鼠肝纤维化的机制初步研究  

An initial research on the mechanism of N-acetyl-L-cysteine against rat liver fibrosis

作　　者：赵宗豪[1] 梅俏[2] 吴军[2] 胡咏梅[2] 徐新华[2] 许建明[2] 

机构地区：[1]安徽医科大学附属省立医院感染病科,合肥230001 [2]安徽医科大学第一附属医院消化内科,合肥230022

出　　处：《安徽医学》2010年第1期5-6,共2页Anhui Medical Journal

基　　金：国家教育部科学技术研究重点课题(编号:02068);安徽省自然科学基金项目(编号:01043904)

摘　　要：目的研究N-乙酰半胱氨酸保护大鼠肝纤维化的机制。方法用四氯化碳(CCl4)制造大鼠肝纤维化模型,同时予100mg·kg-1·d-1N-乙酰半胱氨酸腹腔注射。在造模的第3个月末处死大鼠,用α-SMA单抗标记活化的肝脏星状细胞,并计算其数量,同时进行肝脏纤维化评分。结果N-乙酰半胱氨酸可以显著减少活化的HSC数量,降低CCl4诱导大鼠肝脏纤维化评分。结论N-乙酰半胱氨酸是通过抑制HSC的活化达到保护CCl4诱导的大鼠肝纤维化作用。Objective To study the mechanism of N-acetyl-L-cysteine against rat liver fibrosis. Methods Rat liver fibrosis model was induced by carbon tetrachloride （CC14）, and meanwhile treated with melatonin i.p. at the dose of 100mg/kg body weight. The rats were killed at the end of 3rd month and the activated hepatic stellate c, ells （HSCs） labeled by α- smooth muscle actin （α-SMA） in the liver specimen were counted, the score of liver fibrosis was also measured. Results N-acetyl-L-cysteine decreased the score of rat liver fibrosis induced by CCl4, and the number of activated HSCs was also significantly decreased. Conclusion By inhibiting the activation of HSCs, N-acetyl-L-cysteine ameliorated rat liver fibrosis induced by CCl4.

关 键 词：N-乙酰半胱氨酸 肝纤维化 机制 

分 类 号：R575.2[医药卫生—消化系统]