黄蘑菇深层发酵条件研究  

Conditions in deep submerged fermentation of Armillarialuteo viren

作　　者：周连玉[1] 朱莉莉[1] 邓旭武[1] 姜蓉[1] 

机构地区：[1]青海师范大学生命与地理科学学院,青海西宁810008

出　　处：《湖南农业大学学报（自然科学版）》2010年第1期110-112,共3页Journal of Hunan Agricultural University(Natural Sciences)

摘　　要：为科学开发黄蘑菇资源,采用单因素试验和正交试验研究黄蘑菇的深层发酵条件.结果表明,黄蘑菇发酵的适宜碳源为蔗糖,适宜氮源为牛肉浸膏;黄蘑菇深层发酵的最适培养基为4%蔗糖、0.1%牛肉浸膏、0.10%KH2PO4、0.10%MgSO4;适宜接种的种龄为4d,接种量(移入液体菌种的体积与接种后培养液体积的比)为15%,发酵时间为8d,在此条件下,黄蘑菇菌丝体生物量(每1mL发酵液中收集的菌丝体干重)达1.137mg/mL.Conditions in deep submerged fermenttation of Armillarialuteo viren was studied for exploiting Armillarialuteoviren resources by one-factor experiment. The results showed that the optimum carbon sources was sucrose and the optimum nitrogen sources was sucrose beef extract. The optimum medium by the orthogonal test was sucrose 4%, beef extract 0.1%, KH2PO4 0.10% and MgSO40.10 %. The fermentation conditions were as follows： seed age 4 d, inoculation rate 15% ,culture time 8 d. Under these conditions, myceliun biomass could reach 1.137 mg/mL.

关 键 词：黄蘑菇 发酵条件 菌丝体生物量 

分 类 号：S576[农业科学—作物学]