土茯苓化学成分的分离与鉴定  

Isolation and identification of chemical constituents from stems of Smilax glabra Roxb.

作　　者：吴博[1] 马跃平[1] 袁久志[1] 孙启时[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2010年第2期116-119,共4页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省教育厅科研项目(20060878)

摘　　要：目的对土茯苓的化学成分进行分离和鉴定,为土茯苓药材的质量控制提供依据。方法采用大孔树脂、硅胶、聚酰胺、ODS和Sephadex LH-20柱色谱方法进行分离,根据IR、1H-NMR、13C-NMR等谱学方法对化合物进行结构鉴定。结果从土茯苓中分离得到9个化合物,分别鉴定为β-谷甾醇棕榈酸酯(β-sitosterol palmitate,1)、β-谷甾醇(β-sitosterol,2)、胡萝卜苷(daucosterol,3)、1-棕榈酰基-3-O-β-D-半乳糖基甘油酯(1-O-hexadecanoyl-3-O-β-D-galactop yranosyl-glycerol,4)、落新妇苷(astilbin,5)、槲皮素(quercetin,6)、花旗松素(taxilfolin,7)、槲皮素-4′-O-β-D-吡喃葡萄糖苷(quercetin-4′-O-β-D-pyranglucoside,8)、异落新妇苷(isoastilbin,9)。结论化合物1、4为首次从菝葜属中分离得到,化合物8为首次从该植物中分离得到。Objective To study the chemical constituents of the stems of Smilax glabra Roxb. Methods Compounds were isolated by the silica gel, macroporous resin, polyamide, ODS and Sephadex LH-20 columns, and their structures were identified on the basis of the physicochemical characters and the spectral analysis. Results Nine compounds were obtained and identified as β-sitosterol palmitate（1） ,β-sitosterol （2）, dancosterol （3）, 1-O-hexadecanoyl-3-O-β-D-galactopyranosyl-glycerol （ 4 ）, astilbin （ 5 ）, quercetin （ 6 ）, taxifolin （7）, quercetin-4＇-O-β-D-pyranglucoside （ 8 ）, isoastilbin （ 9 ）. Conclusions Compounds 1 and 4 are obtained from Smilax L. for the first time, Compound 8 is isolated from this plant for the first time.

关 键 词：土茯苓 化学成分 结构鉴定 β-谷甾醇棕榈酸酯 1-棕榈酰基-3-O-β-D-半乳糖基甘 油酯 

分 类 号：R914[医药卫生—药物化学] R284[医药卫生—药学]