长雄野生稻紫色柱头性状的遗传和基因定位研究  

A genetic study on the purple stigma genes and their locations in Oryza longistaminata

作　　者：陈志伟[1] 邓伟[2] 李飞[2] 周家武[3] 李静[3] 徐鹏[3] 邓先能[3] 胡凤益[3] 王荔[2] 陈善娜[1] 陶大云[3] 

机构地区：[1]云南大学生命科学学院,云南昆明650091 [2]云南农业大学农学与生物技术学院,云南昆明650201 [3]云南省农业科学院粮食作物研究所,云南昆明650205

出　　处：《云南大学学报（自然科学版）》2010年第1期103-107,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省自然科学基金资助项目(2006C0063M;2006PG09);科技部973前期专项资助项目(2006CB708207)

摘　　要：由花青素合成代谢形成的紫色柱头性状在包括长雄野生稻在内的AA基因组野生稻中较为普遍.为研究长雄野生稻中的紫色柱头性状,以具无色柱头的亚洲栽培稻品种RD23为轮回亲本与紫色柱头的长雄野生稻进行回交,经胚挽救和多代连续选择,获得3个柱头颜色有分离的BC6F1定位群体.这些群体中,柱头颜色均适合1(紫色)∶1(无色)的分离比例,表明紫色柱头性状受一对显性核基因控制.通过微卫星标记分析,将控制紫色柱头的基因定位在水稻第6染色体上,距标记RM253,RM111和RM6917分别为2.5,0cM和4.4cM.对比已发表的紫色柱头基因座位,它可能与来自亚洲栽培稻的Ps-4(t)基因等位,所以暂命名为Ps-4(t).The purple stigmas, which were caused by the metabolism of anthocyanins, were normal among all AA genome wild rice species including Oryza longistaminata. To study the purple stigmas in O. longistaminata, backcrossing was applied between the donor parent O. longistaminata with achromatic stigmas and recurrent parent RD23 with purple stigmas, and after embryo rescue and consecutive backcrossing three BC6F1 populations that showed character segregation in stigma color were finally got. In all BC6F1 populations, the segregation raito of purple stigma to achromatic stigma was 1： 1, suggesting that the purple stigma was controlled by a pair of dominant allele. An analysis using microsatellite markers （SSR） demonstrated that the target gene located on the No. 6 chromosome which was 2.5 cM, 0 cM and 4.4 cM from RM253, RM111 and RM6917,respectively. After comparing its position and effect to those published data, this gene might be allelic to Ps -4 （t）, which was identified from O. sativa.

关 键 词：紫色柱头 长雄野生稻 亚洲栽培稻 渗入 分子定位 ps-4(t) 

分 类 号：Q32[生物学—遗传学]