蝉拟青霉多糖体外对乙型肝炎病毒复制的抑制作用  

Effect of Paecilomyces cicadae polysaccharide on duplication of hepatitis B virus in HepG2.2.15 cell strain

作　　者：卓佳[1] 杨介钻[2] 金丽琴[3] 陈柏坤[1] 

机构地区：[1]温州医学院生命科学学院,浙江温州3250233 [2]浙江大学医学院附属第一医院,浙江杭州310003 [3]温州医学院生物化学教研室,浙江温州325023

出　　处：《中国病理生理杂志》2010年第2期327-332,共6页Chinese Journal of Pathophysiology

基　　金：浙江省教育厅科研基金资助项目(No.20020471);温州市"551"人才基金资助项目

摘　　要：目的:探讨蝉拟青霉多糖(PcPS)体外对乙型肝炎病毒(HBV)的抑制作用及对HepG2.2.15细胞表达Toll样受体4(TLR4)mRNA的影响。方法:以不同浓度PcPS与HepG2.2.15细胞株共培养,以拉米夫定(LMV)为阳性对照,采用四甲基偶氮唑盐(MTT)法和ELISA法,检测PcPS对HepG2.2.15细胞毒性作用及其分泌HBsAg、HBeAg的情况。实时荧光定量PCR分析PcPS对HepG2.2.15细胞表达与分泌HBV-DNA和TLR4 mRNA表达的影响。结果:蝉拟青霉多糖在体外能有效抑制HepG2.2.15细胞分泌HBsAg、HBeAg,最大抑制率分别为44.8%、31.0%;对HepG2.2.15细胞内HBV-DNA复制和TLR4 mRNA表达均有一定抑制作用。结论:一定浓度的蝉拟青霉多糖在体外具有显著抑制HBV的复制作用。AIM： To investigative the inhibitory effects of Paecilomyces cicadae polysaccharide （PcPS） against HBV in vitro, and the effects on the Toll like receptor 4 （TLR4） mRNA expression in HepG2. 2. 15 cell strain. METHODS ： HepG2. 2. 15 cell strain was co - cultured in vitro with PcPS in different concentrations, and lamivudine （LMV） was applied as positive control. MTT assay was employed to detect the cytotoxicities of PcPS in vitro, when the HepG2. 2. 15 ceils was used as target cells. The effects of PcPS on the secretion of HBsAg and HBeAg were assayed by ELISA method. Fluorescence quantitative - PCR （ FQ - PCR） was used to detect the inhibitory effects of PcPS on the content of HBV - DNA and TLR4 mRNA in HepG2. 2. 15 cells. RESULTS： The inhibitory effects of PcPS on the HBsAg and HBeAg were observed and the maximum inhibitory ratio up to 44. 8% and 31.0% , respectively. The same inhibitory effects of PcPS on the HBV -DNA replication and TLR4 mRNA expression in HepG2. 2. 15 cells were also found. CONCLUSION： A certain concentration of PcPS significantly inhibits HBV replication in vitro.

关 键 词：蝉拟青霉多糖 肝炎病毒 乙型 TOLL样受体4 

分 类 号：R575.1[医药卫生—消化系统]