豚鼠糖尿病性膀胱病逼尿肌中c-kit mRNA和蛋白的表达变化及意义  

Expression and significance of c-kit messenger ribonucleic acid and c-kit protein in diabetic cystopathy in guinea pig

作　　者：李云飞[1] 丁国富[1] 蔡志强[1] 范勇洪[1] 王勤章[1] 

机构地区：[1]石河子大学医学院第一附属医院泌尿外科,新疆石河子832000

出　　处：《中国病理生理杂志》2010年第2期345-348,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30860281)

摘　　要：目的:检测豚鼠糖尿病性膀胱病(DCP)逼尿肌中酪氨酸蛋白激酶生长因子受体(c-kit)mRNA和蛋白的表达水平,探讨c-kit表达与DCP的关系及其发病机制。方法:60只豚鼠随机分成对照组20只,实验组40只,实验组采用链脲佐菌素(STZ)建立糖尿病豚鼠模型,2组豚鼠饲养10周后运用尿动力学检测并将实验组筛选出DCP组和糖尿病性非膀胱病(NDCP)组,应用RT-PCR、激光共聚焦显微镜技术分别检测各组膀胱组织c-kit mRNA和蛋白的表达。结果:DCP组c-kit mRNA表达显著低于对照组(P<0.01)及NDCP组(P<0.05),相对平均吸光度值分别为4.65±0.47、5.66±0.54、5.54±1.28。糖尿病性膀胱病组c-kit蛋白表达明显低于对照组(P<0.01)与NDCP组(P<0.01),c-kit蛋白荧光值分别为548.69±48.51、844.67±59.24、856.52±53.03。结论:豚鼠DCP逼尿肌中c-kit mRNA、c-kit蛋白表达减少,导致c-kit信号通路异常,从而使Cajal样细胞在膀胱中的功能减弱而导致逼尿肌功能障碍发生DCP,因此c-kit表达异常可能是DCP的发病机制之一。AIM ： To determine the expression of c - kit mRNA and protein in the bladders in guinea pigs with diabetic cystopathy （DCP） and to explore the correlation and mechanisms between c - kit expression and DCP. METHODS ： Sixty guinea pigs were divided randomly into control group （ n= 20） and experimental group （ n = 40）. The guinea pigs in experimental group were injected with streptozotocin （STZ） to induce diabetes mellitus. After fed for 10 weeks, the animals in both groups were tested with urodynamics, and the guinea pigs in experimental group were divided into the subgroups of DCP and the diabetic no - cystopathy （NDCP） group according to the results of urodynamics, mRNA expression of c - kit was detected by reverse transcription polymerase chain reaction （ RT - PCR） and protein expression of c - kit was tested and analyzed by laser scanning confocal microscope, RESULTS： Decreased expression of c - kit mRNA was observed in DCP group compared to control and the NDCP group. The ratio of c - kit mRNA and GAPDH was 5.66 ± 0. 54 in controls （ P 〈 0. 05 ）, 5.54 ± 1.28 in NDCP group （ P 〈 0.05） and 4. 65 ±0. 47 in DCP group, c - kit protein expression significantly declined in DCP group. The mean value of fluorescence intensity was 856. 52 ± 53.03 in control group, 844, 67 ±59. 24 in NDCP group and 548. 69 ±48.51 in DCP （P 〈0. 01 ）. CONCLUSION： The declined expression of c - kit gene at transcription and translation levels destroys the SCF/c - kit signal pathway, leading to the dysfunction of Cajallike cells in DCP guinea pig, so the abnormal expression of c -kit gene is involved in the Dathogenesis of DCP.

关 键 词：CAJAL细胞 糖尿病膀胱病 C-KIT SCF/c-kit信号通路 

分 类 号：R363[医药卫生—病理学]