H_2O_2对大鼠骨骼肌卫星细胞凋亡和线粒体膜电位的影响及EPO的保护作用  

Effects of H_2O_2 on apoptosis of skeletal muscle satellite cell and mitochondrial membrane potential

作　　者：罗林杰[1,2] 李江华 董少红[1,2] 陈科奇 

机构地区：[1]暨南大学第二临床学院 [2]深圳市人民医院心内科,广东深圳518020

出　　处：《中国病理生理杂志》2010年第2期357-361,共5页Chinese Journal of Pathophysiology

基　　金：深圳市科技局资助项目(No.200602004)

摘　　要：目的:探讨过氧化氢(H2O2)对大鼠骨骼肌卫星细胞(SMSC)凋亡和线粒体膜电位(MMP)的影响以及促红细胞生成素(EPO)的保护作用。方法:取体外培养的骨骼肌卫星细胞,随机分为正常对照组、H2O2组、H2O2+EPO组,采用流式细胞仪(FCM)检测细胞凋亡率和MMP的平均荧光强度,Hoechst33258染色后荧光显微镜观察凋亡细胞的形态学变化。结果:H2O2组凋亡发生率最高,为(22.13±1.79)%,经10、20、40kU/L浓度的EPO预处理后凋亡率分别为(16.47±2.53)%、(4.97±0.55)%、(2.93±0.47)%;H2O2干预组MMP最低,为9.70±0.09,经10、20、40kU/L浓度的EPO预处理后MMP分别为12.67±0.32、27.90±0.66、44.53±0.93,对照组凋亡率为(1.93±0.57)%,MMP为51.37±0.64;在H2O2干预组和低剂量EPO预干预组,Hoechst33258染色呈现明显的凋亡征象。结论:EPO可抑制H2O2诱导的细胞凋亡并稳定线粒体膜电位。AIM： To observe the effects of H2O2 on apoptosis of skeletal muscle satellite cells （SMSC）and mitochondrial membrane potential （MMP）, and protective effect of erythropoietin（EPO）. METHODS： SMSC in vitro were divided into three groups： H2O2 group, H2O2 ＋ EPO group and control. Apoptosis rate and the means were obverted by monofluorescence flow cytometry. The morphological change of apoptosis cells were observed under fluorescence microscopy after Hoechst 33258 staining. RESULTS： The ceils in H2O2 group show the highest apoptosis rate （22. 13 ±1.79）%. In H2O2 ＋EPO group, apoptosis rate were （16.47±2.53）%, （4.97±0.55）% and （2.93±0.47）% according to the EPO treated levels （10, 20 or 40 kU/L）, respectively. MMP level in H2O2 group was the lowest 9. 70±0. 09. MMP levels in H2O2 ＋ EPO group were 12. 67±0. 32, 27.90 ±0. 66, 44. 53±0. 93, respectively according to the EPO treated levels （ 10, 20 or 40 kU/L）. In control group, apoptosis rate was 1.93 ±0. 57 and MMP was 51.37±0. 64. In H2O2 group and H2O2 ＋ low dosage EPO group, Hoechst 33258 staining showed obvious apoptosis. CONCLUSION： EPO inhibits the apoptosis induced by H2O2 and stabilizes the MMP, which is related to the dosage of EPO.

关 键 词：过氧化氢 骨骼肌卫星细胞 细胞凋亡 线粒体膜电位 促红细胞生成素 

分 类 号：R364.2[医药卫生—病理学] R541.4[医药卫生—基础医学]