溃疡性结肠炎患者血浆P-选择素、血小板膜糖蛋白Ⅰb/Ⅸ/Ⅴ复合物与疾病活动性的关系  

Relationship of Serum P-selectin and Platelet Membrane Glycoprotein Ⅰb/Ⅸ/Ⅴ Complex with Activity of Ulcerative Colitis

作　　者：唐庆[1] 范恒[1] 沈霖[1] 张丽娟[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科,武汉430022

出　　处：《中西医结合研究》2010年第1期1-3,共3页Research of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金项目(No.30772878)

摘　　要：目的探索溃疡性结肠炎(ulcerative colitis,UC)患者血浆P-选择素(P-selectin)、血小板膜糖蛋白(GP)Ⅰb/Ⅸ/Ⅴ复合物与疾病活动性的关系。方法选择28例UC活动期、16例UC缓解期患者和26例健康志愿者,采用ELISA法检测血浆P-selectin,比浊法检测血小板最大聚集率(MAR),并采用流式细胞仪检测GPⅠb/Ⅸ/Ⅴ复合物。结果活动期UC患者血浆P-selectin、血小板MAR较缓解期患者及健康者明显升高(P<0.01或P<0.05),而GPⅠb/Ⅸ/Ⅴ复合物的表达显著降低(P<0.01或P<0.05)。结论溃疡性结肠炎患者存在血小板激活,血浆P-selectin和GPⅠb/Ⅸ/Ⅴ复合物作为血小板激活的标志物,与疾病的活动程度相关,可以作为UC活动期的指标。Objective To explore the relationship of serum P-selectin and platelet membrane glycoprotein Ⅰb/Ⅸ/Ⅴ complex with activity of ulcerative colitis（UC）. Methods Twenty-eight UC patients in active phase,sixteen catabasis UC patients and twenty-six health volunteers were involved in this study. Serum P-seleetin was detected by ELISA method. The maximum agglutination rat（MAR）of platelets was assayed by platelet aggregometer. GP Ⅰb/Ⅸ/Ⅴ complex was detected by flow cytometry. Results Compared with eatabasis group and normal group,the level of serum P-seleetin and MAR of platelets in active phase group increased significantly（P〈0.01 or P〈0.05）, while the level of GP Ⅰb/Ⅸ/Ⅴ complex decreased obviously（P〈0.01 or P〈0.05）. Conclusion It is suggested that platelet activation phenomenon existed in UC patients. Serum P-selectin and platelet membrane glycoprotein Ⅰb/Ⅸ/Ⅴ complex could be regarded as markers of platelet activation and correlate with the degree of activity of UC,and those can be considered as markers of UC activity.

关 键 词：溃疡性结肠炎 P-选择素 血小板膜糖蛋白Ⅰb/Ⅸ/Ⅴ 

分 类 号：R574.62[医药卫生—消化系统]