一株产脂肽类生物表面活性剂菌株的分离及代谢产物分析  

Isolation and Metabolite Analysis of A Strain Producing Lipopeptide Biosurfactant

作　　者：罗剑波[1] 吴卫霞[2] 张凡 佘跃惠[1] 

机构地区：[1]长江大学化学与环境工程学院,湖北荆州434023 [2]新疆吐哈油田公司温米采油厂采油工程室,新疆鄯善838202 [3]北京百奥赛科技研究中心,北京100085

出　　处：《化学与生物工程》2010年第2期46-49,共4页Chemistry & Bioengineering

基　　金：国家863计划资助项目(2008AA06Z204)

摘　　要：采用多次富集培养、血平板筛选方法,从新疆克拉玛依油田油水样中分离得到产生物表面活性剂菌株L1。该菌株与已培养的土壤芽孢杆菌(Brevibacillus agri)的16S rDNA序列同源性达到99%;其代谢产物具有降低表面张力的作用,可以将发酵液表面张力从最初的69.56 mN.m-1降到29.36 mN.m-1;菌株代谢产物经薄层层析分析初步鉴定为脂肽类生物表面活性剂,红外光谱定性该生物表面活性剂属于环脂肽类表面活性剂。After multiple rounds of enrichment,blood-plate treatment, strain L1 producing lipopeptide biosurfactant was isolated from the oil water sample of the Kelamayi Oilfield. Phylogenetic rDNA gene sequence indicated that strain L1 was closest to Brevibacillus agri with 99 % tension of cultured fluid of strain L1 reduced from 69.56 mN · m^-1 to 29.36 mN · m^- 1 , identified preliminarily as lipopeptide biosurfactant by thin layer chromatography and as surfactant by FTIR spectroscopy. analysis based on 16S similarity. The surface and the metabolite was a cyclic lipopetide biosurfactant by FTIR spectroscopy.

关 键 词：脂肽类生物表面活性剂 代谢产物 分离 血平板筛选法 16SrDNA 土壤芽孢杆菌 

分 类 号：TQ423[化学工程] TQ920.9[轻工技术与工程—发酵工程]