荧光原位杂交和免疫组织化学方法检测乳腺癌组织中人类表皮生长因子受体2基因状态差异及其特征分析  

Differences of HER-2 status in breast cancer tissues detected by FISH and IHC and its related characteristics

作　　者：唐录英[1] 何丹[1] 周静[1] 冯智英[1] 张志刚[1] 余丹丹[2] 任泽舫[2] 邵春奎[1] 

机构地区：[1]中山大学附属第三医院病理科,广州510630 [2]中山大学公共卫生学院卫生统计与流行病学系,广州510080

出　　处：《中华乳腺病杂志（电子版）》2010年第1期41-44,共4页Chinese Journal of Breast Disease(Electronic Edition)

基　　金：卫生部科学基金资助项目(WKJ2007-3-001)

摘　　要：目的比较荧光原位杂交(fluorescence in situ hybridization,FISH)和免疫组织化学(immunohistochemistry,IHC)检测乳腺癌组织中人类表皮生长因子受体2(human epidermal growth factor receptor 2,HER-2)基因状态差异,分析其相关特征。方法回顾性收集51例中山大学附属第三医院2007年10月至2008年9月间乳腺浸润性导管癌组织标本和相关信息,分别采用IHC和FISH法检测HER-2蛋白表达情况和基因扩增状态,比较两种方法的结果差异,用Fisher精确概率检验分析这种差异的相关因素。结果在51例标本中,FISH检测乳腺癌组织中HER-2基因阴性32例(62.75%,32/51),阳性19例(37.25%,19/51);IHC检测乳腺癌组织中HER-2为(-)和(+)者的FISH检测均为阴性(21例);12例IHC检测HER-2为(++),其中3例FISH检测为阳性,其余9例FISH检测为阴性;18例IHC检测HER-2为(+++)者仍有2例FISH检测结果阴性。月经状态和雌激素受体(ER)表达与IHC检测HER-2阳性病例的FISH阳性结果具有显著联系(P值分别为0.023和0.007),即在IHC检测HER-2阳性[(++)和(+++)]的病例中,绝经前女性较绝经期女性以及ER阴性者较阳性者更可能为FISHHER-2阳性(有HER-2基因扩增)。结论本研究的结果有助于提高IHC判断HER-2基因扩增的准确性。Objective To compare the differences of human epidermal growth factor receptor 2 （HER-2） status in breast cancer tissues detected by fluorescence in situ hybridization （FISH） and immunohistochemistry （IHC）, and search for its related characteristics. Methods Fifty-one samples of ductal invasive breast cancer tissues were recruited retrospectively from Oct. 2007 to Sep. 2008 in our hospital. FISH and ICH were applied to detect HER-2 expression and gene amplification. Fisher＇s exact probability test was used to identify the related factors of the differences. Results Among the 51 samples, FISH showed that HER-2 was negative in 32 cases （62.75%,32/51）, and positive in 19 cases （37.25%,19/51）. Twenty-one cases with HER-2 （-） or （4-） by IHC were all HER-2 negative by FISH. Among the 12 cases with HER-2 （＋＋） by IHC, 3 cases were HER-2 positive and 9 cases HER-2 negative by FISH. Among the 18 cases with HER-2 （ ＋＋＋ ） by IHC, 2 cases were HER-2 negative by FISH. Menstrual status and estrogen receptor （ER） expression were statistically significantly associated with FISH positive result among IHC positive cases （P= 0. 023, P= 0.007 respectively）. Among the patients with HER-2 （ ＋＋- ） or （ ＋＋＋） by IHC, premenopausal and ER negative patients were more likely to be HER-2 positive by FISH （meaning HER-2 gene amplification） compared with the postmenopausal and ER positive patients respectively. Conclusion The results of this study helps to raise judging the IHC accuracy in detecting HER-2 amplification.

关 键 词：乳腺肿瘤 免疫组织化学 荧光原位杂交 人类表皮生长因子受体2 

分 类 号：R737.9[医药卫生—肿瘤]