HPLC法同时测定不同产地满山红中4种黄酮成分的含量  

HPLC simultaneous determination of four flavonoids in Rhododendron dauricum L

作　　者：梁泰刚[1] 孟旭鹏[1] 赵承孝[1] 班树荣[1] 李青山[1] 

机构地区：[1]山西医科大学药学院,太原030001

出　　处：《药物分析杂志》2010年第2期279-281,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：山西省科技攻关项目(NO.2006031201-01);山西医科大学校青年基金资助项目(NO.2006011)

摘　　要：目的:建立同时测定满山红药材中金丝桃苷、槲皮素、山柰酚和杜鹃素含量的高效液相色谱方法。方法:采用Agilent Zorbax SBC18色谱柱(4.6mm×150mm,5μm);流动相为甲醇(A)-0.2%磷酸水溶液(B),梯度洗脱(0～20min,35%A→60%A;20～40min,60%A→70%A),流速0.8mL.min-1;检测波长为360nm(0～29min,测定金丝桃苷、槲皮素和山柰酚)和299nm(29～40min,测定杜鹃素);柱温25℃。结果:金丝桃苷、槲皮素、山柰酚和杜鹃素线性范围分别为6.40～160.0μg.mL-1(r=0.9995),3.33～83.33μg.mL-1(r=0.9993),0.80～20.0μg.mL-1(r=0.9998),2.67～66.67μg.mL-1(r=0.9996);金丝桃苷、槲皮素、山柰酚和杜鹃素回收率(n=3)分别为96.5%～99.2%(RSD为1.2%～1.6%),96.6%～98.9%(RSD为0.9%～2.8%),96.4%～101.7%(RSD为2.0%～2.9%),95.9%～100.4%(RSD为0.4%～2.2%)。结论:该方法简便快速,结果准确可靠,可用于中药满山红的质量控制。Objective：To establish an HPLC method for simultaneous determination of hyperoside,quercetin,kaempferol and farrerol in Rhododendron dauricum.Methods：The separation was performed on an Agilent Zorbax SB C18column（4.6 mm×150 mm,5 μm） by gradient elution（0-20 min,35% A→60% A 20-40 min,60% A→70% A） using methanol（A） and 0.2% phosphoric acid（B） as the mobile phase.The flow rate was 0.8 mL/min-1 The detection wavelength was 360 nm（0-29 min） for hyperoside,quercetin,kaempferol,and 299 nm（29-40 min） for farrerol with column temperature at 25 ℃.Results：The linear ranges of hyperoside,quercetin,kaempferol and farrerol was 6.40-160.0 μg/mL-1（r=0.9995）,3.33-83.33 μg/mL-1（r=0.9993）,0.80-20.0 μg/mL-1（r=0.9998）,and 2.67-66.67 μg/mL-1（r=0.9996）,respectively The average recoveries（n=3） was 96.5%-99.2% with RSD of 1.2%-1.6%,96.6%-98.9% with RSD of 0.9%-2.8%,96.4%-101.7% with RSD of 2.0%-2.9%,95.9%-100.4% with RSD of 0.4%-2.2% respectively.Conclusion：The method is simple,accurate and can be used for quality control of Rhododendron dauricum.

关 键 词：高效液相色谱法 满山红 金丝桃苷 槲皮素 山柰酚 杜鹃素 

分 类 号：R917[医药卫生—药物分析学]