IL12基因多态性与原发性肝细胞肝癌遗传易感性的关联研究  

Association between IL12 polymorphism and primary hepatocellular carcinoma in Chinese population

作　　者：刘莉[1,2] 刘政[1] 徐研[3] 董静[3] 沈洪兵[3] 胡志斌[3] 

机构地区：[1]南京医科大学第二附属医院消化科,江苏南京210011 [2]南通市肿瘤医院内科,江苏南通226361 [3]南京医科大学肿瘤中心流行病与卫生统计教研室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2010年第1期34-38,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：卫生部科学研究基金项目(WKJ2005-2-027)

摘　　要：目的:探讨IL12A和IL12B基因多态性与江苏人群原发性肝细胞肝癌的遗传易感性的关系。方法:采用病例对照研究,对明确诊断的原发性肝细胞肝癌新发病例538例按年龄和城乡频数匹配正常对照535例,以聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法对IL12A基因的rs568408(3'UTRG>A)、rs2243115(5'UTRT>G)和IL12B基因的rs3212227(3'UTRA>C)进行多态性检测,比较不同基因型与原发性肝细胞肝癌发病风险的关系。结果:携带IL12Ars568408变异等位基因的个体(rs568408AG/AA)发生原发性肝细胞肝癌的风险较携带野生纯合子(rs568408GG)增加了35%(调整OR=1.35,95%CI=1.03～1.79)。进一步对IL12Ars568408位点的作用按年龄、性别、吸烟情况、饮酒情况、HBsAg和抗HCVAb携带情况进行分层分析,结果发现rs568408位点对原发性肝细胞肝癌发病风险的影响在男性和有饮酒史患者中更为显著(调整OR分别为1.46和1.53,95%CI分别为1.08～1.98和1.07～2.18)。结论:IL12A rs568408可能影响江苏人群原发性肝细胞肝癌发病风险。Objective：To investigate the association between potentially functional polymorphisms in IL12A and IL12B and risk of primary hepatocellular carcinoma（PHC）.Methods：We genotyped three IL12A polymorphisms rs568408（3＇UTR G〉A）,rs2243115（5＇UTR T〉G） and IL12B rs3212227（3＇UTR A〉C） using the RFLP-and PIRA-PCR assays in a case-control study of 538 HCC cases and 535 cancer-free controls.Results：The IL12A rs568408 GA / AA variant genotypes were associated with a significantly increasing risk of PHCadjusted odds ratio（OR）=1.35,95% confidence interval（CI）=1.03～1.79,compared with their corresponding wild-type homozygotes.In the stratified analysis,rs568408 genetic effect was more pronounced in male and drinker（adjusted OR=1.46,95% CI= 1.08～1.98 for male;adjusted OR=1.53,95% CI=1.07～2.18 for drinker）.Conclusion：Our findings indicate that IL12A rs568408 may contribute to the risk of PHC.

关 键 词：IL12 单核苷酸多态性 原发性肝细胞肝癌 分子流行病学 

分 类 号：R365[医药卫生—病理学]