罗丹明123人工抗原的合成及抗体的酶联免疫检测  

Synthesis of Rhodamine 123 Artificial Antigen and ELISA Determination of Rhodamine B

作　　者：王庭欣[1] 赵志磊[1] 李小亭[1] 

机构地区：[1]河北大学质量技术监督学院,河北保定071002

出　　处：《食品科学》2010年第2期211-213,共3页Food Science

基　　金："十一五"国家科技支撑计划项目(2006BAK02A17)

摘　　要：为建立罗丹明B(rhodamine B)的免疫分析方法,采用戊二醛法,将半抗原罗丹明123与载体牛血清白蛋白(BSA)及卵清蛋白(OVA)偶联制备免疫抗原R123-B S A和包被抗原R123-OVA。经动物免疫实验证实人工抗原合成,并制备抗罗丹明123的多克隆抗体,此抗体同时能够检测食品中的罗丹明B,且最低检测限为0.001ng/mL。For establishing an enzyme-linked immunosorbent assay （ELISA） method for rhodamine B determination, a complete antigen R 123-BS A and a coating antigen R 123-OVA were prepared by coupling rhodamine 123 with the carder proteins of bovine serum albumin （BSA） and ovalbumin （OVA） using glutaraldehyde method, respectively. The synthesis of rhodamine123 artificial antigens was confirmed by animal experiments. A novel anti-rhodamine123 polyclonal antibody was prepared, which was successfully applied to the determination of rhodamine B in food with a limit of detection of 0.001 ng/mL. The development of anti-rhodamine123 antibody will provide a valuable tool for the determination of rhodamine B in food seasoning products.

关 键 词：罗丹明123 罗丹明B 戊二醛法 人工抗原 酶联免疫检测 

分 类 号：R446.61[医药卫生—诊断学]