2-羟丙基三甲基氯化铵脱乙酰壳多糖和碱性成纤维细胞生长因子对白细胞介素-1β和肿瘤坏死因子-α的影响  

Effect of 2-hydroxy-propyl trimethyl ammonium chloride chitosan and basic fibroblast growth factor on production of interleukin-1β and tumor necrosis factor-α

作　　者：吉秋霞[1] 袁昌青[1] 徐全臣[1] 于新波[1] 

机构地区：[1]青岛大学医学院附属医院口腔科,山东青岛266003

出　　处：《国际口腔医学杂志》2010年第1期17-20,共4页International Journal of Stomatology

基　　金：山东省卫生厅青年科研基金资助项目(2007QZ021);青岛市科技计划基金资助项目(06-2-2-6-nsh-2)

摘　　要：目的观察在脂多糖(LPS)刺激下,2-羟丙基三甲基氯化铵脱乙酰壳多糖(HTCC)和碱性成纤维细胞生长因子(bFGF)对人牙周膜成纤维细胞(hPDLF)分泌白细胞介素(IL)-1β和肿瘤坏死因子(TNF)-α的影响。方法在50mg·L-1的LPS刺激下,采用酶联免疫吸附测定观察质量浓度为1g·L-1的HTCC和100μg·L-1的bFGF对50mg·L-1的LPS刺激hPDLF分别于24、48和72h分泌IL-1β和TNF-α的质量浓度变化。结果1g·L-1的HTCC具有促进LPS刺激hPDLF分泌IL-1β和TNF-α的作用,分泌量48h达高峰。在100μg·L-1的bFGF的作用下,LPS介导的hPDLF分泌IL-1β和TNF-α的质量浓度明显下降。HTCC与bFGF联合较单独应用时,IL-1β和TNF-α的质量浓度下降显著(P≤0.001)。结论HTCC对LPS介导hPDLF分泌IL-1β和TNF-α具有促进作用,HTCC与bFGF联合应用能有效地抑制IL-1β和TNF-α的分泌。Objective The aim of this study was to observe the effect of 2-hydroxy-propyl trimethyl ammonium chloride chitosan（HTCC） and basic fibroblast growth facto（rbFGF） on production of interleukin（IL）-1β and tumor necrosis factor（TNF）-α in human periodontal ligament fibroblas（thPDLF） stimulated by lipopolysaccharide（LPS）.Methods The levels of IL-1β and TNF-α in hPDLF stimulated by 50 mg·L-1 LPS was observed and the effects of 1 g·L-1 HTCC and 100 μg·L-1 bFGF on the IL-1β and TNF-α were determined by enzyme-linked immunosorbent assay.Results 1 g·L-1 HTCC can stimulate the level of IL-1β and TNF-α in hPDLF stimulated by LPS and the level of cytokines were highest at 48 h.100 μg·L-1 bFGF can decrease the IL-1β and TNF-α.The level of IL-1β and TNF-α in hPDLF stimulated by LPS was statistically decreased by association of HTCC and bFGF（P≤0.001）.Conclusion HTCC can increase the production of IL-1β and TNF-α in hPDLF stimulated by LPS.And HTCC associated with bFGF can effectively inhibit the level of IL-1β and TNF-α of hPDLF.

关 键 词：2-羟丙基三甲基氯化铵脱乙酰壳多糖 人牙周膜成纤维细胞 脂多糖 白细胞介素-1Β 肿瘤坏死因子-α 

分 类 号：R78[医药卫生—口腔医学]