梅毒抗体不同检测方法在献血者血液筛查中应用探讨  

Exploring the Significance of Treponema Pallidum Antibody Detection in Blood Screening

作　　者：贾幼珍 高仕卿 徐力东[1] 秦春怡 

机构地区：[1]河北省唐山市中心血站,唐山063000 [2]河北省唐山市中医院,唐山063000

出　　处：《中国医药导刊》2010年第1期120-120,122,共2页Chinese Journal of Medicinal Guide

摘　　要：目的:比较梅毒螺旋体抗体ELISA与RPR、TPPA试验,并对检测结果进行综合分析,选择适合采供血机构筛检梅毒的方法。方法:选用ELISA方法在无偿献血者筛检中产生的50例梅毒抗体阳性标本,分别进行梅毒螺旋体抗体酶联免疫试验(TP-ELISA)、血浆反应素环状卡片试验(RPR)以及梅毒螺旋体血球凝集试验(TPPA),将检测结果进行综合分析。结果:ELISA-1、ELISA-2、RPR法的灵敏度分别为100%、100%、44%;假阳性率分别为4%、60%、0;漏检率分别为0、0、56%。TPPA法与RPR法检测结果之间有显著性差异,TPPA法与ELISA-2法检测结果之间有显著性差异,TPPA法与ELISA-1法之间无显著性差异。结论:ELISA-1法适用于大批量的血液筛查,但此法可能会造成检测结果出现一定程度的假阳性,采用TPPA法再进行确证,有利于血液质量安全。Objective：To compare the Treponema pallidum antibody ELISA and RPR. TPPA test and a comprehensive analysis of test results, select syphilis screening for blood collection and supply agencies.Methods：ELISA method in screening blood donors in 50 cases of syphilis have the antibody-positive specimens, respectively Treponema pallidum antibody ELISA （TP-ELISA）, plasma-response test card ring （RPR） and the Treponema paltidum hemagglutination Tesl （TPPA）, will conduct a comprehensive analysis of test results.Results： ELISA-1, ELISA-2, RPR sensitivity of the method were 100%, 100%. 44%： false positive rates were 4%, 60%, 0; missed were 0. 0, 56%.TPPA Law and the Law RPR test results have significant differences.TPPA law and ELISA-2 assay results were significantly different, TPPA law and ELISA-I between the law was no significant difference. Conelusion：ELISA- l law applicable to high-volume blood screening, but this method may cause the results of testing a certain degree of false positives. TPPA method used to conduct a confirmation that is conducive to the quality and safety of the blood.

关 键 词：梅毒螺旋体 检测 ELISA RPR TPPA 

分 类 号：R446.62[医药卫生—诊断学]