用清除羟自由基法评价竹叶提取物抗氧化能力  

Detection of Antioxidative Capacity of Bamboo Leaf Extract by Scavenging Hydroxyl Free Radical

作　　者：郭雪峰[1] 岳永德[1] 孟志芬[2] 汤锋[1] 王进[1] 姚曦[1] 荀航[1] 孙嘏[1] 

机构地区：[1]国际竹藤网络中心,国家林业局竹藤科学与技术重点开放实验室,北京100102 [2]河南科技学院化学化工学院,河南新乡453003

出　　处：《光谱学与光谱分析》2010年第2期508-511,共4页Spectroscopy and Spectral Analysis

基　　金：国家“十一五”科技支撑项目(2006BAD19B08);国际竹藤网络中心基本科研业务费专项资金(1632008003)资助

摘　　要：进行了Fe2+与邻二氮菲生成红色配合物的吸收光谱,抗氧化剂TBHQ及竹叶提取物样品对清除羟自由基能力的研究。分光光度法测定抗氧化剂清除羟自由基能力的测定波长为509.1 nm。以IC50值(清除率为50%时,抗氧化剂的浓度值)作为评价抗氧化剂清除羟自由基能力的指标,测得合成抗氧化剂和效果最好竹叶提取物样品IC50值分别为0.040(TBHQ),0.378(M20),0.323(M40),0.334(M60),M20,M40,M60等竹叶提取物可以作为天然抗氧化剂进行开发。By studying on absorption spectrum of red compound coming from the reaction system of Fe2＋ and 1,10-phenanthro- line and the capacity of antioxidant TBHQ and bamboo leaf extract for scavenging hydroxyl free radical, some results were drawn as follows： the determining wavelength of bamboo leaf extract for scavenging hydroxyl free radical by spectrophotometric method is 509. 1 nm, and IC50 （the value of antioxidant concentration at scavenging half of hydroxyl free radical）was used as the index to evaluate scavenging capacity. The determined IC50 values were TBHQ（0. 040）, M20（0. 378）, M40（0. 323）, M60（0. 334）, and bamboo leaf extract could be used as natural antioxidant.

关 键 词：竹叶提取物 羟自由基 清除率 IC50值 

分 类 号：TS201.2[轻工技术与工程—食品科学]