葛花鸢尾异黄酮对SMMC-7721肝癌细胞增殖的抑制作用  

Effect of tectorigenin from Pueraria flos on proliferation of hepatocarcinoma SMMC-7721 cells in vitro

作　　者：汪新亮[1] 吴基良[2] 郑敏[1] 赵骥[1] 

机构地区：[1]咸宁学院中心实验室,咸宁437100 [2]咸宁学院药理学教研室,咸宁437100

出　　处：《中国新药杂志》2010年第2期168-171,共4页Chinese Journal of New Drugs

摘　　要：目的：研究葛花中的鸢尾异黄酮对SMMC-7721人肝癌细胞株增殖的抑制作用。方法：将野葛花的甲醇提取物用盐酸加热水解得到葛花总异黄酮，总异黄酮经硅胶柱层析分离获得鸢尾异黄酮。SMMC-7721人肝癌细胞培养至对数生长期后，加入不同浓度鸢尾异黄酮进行干预，在倒置显微镜下观察细胞形态的变化；采用四甲基偶氮唑（MTT，methyl thiazolyl tetrazolium）法分析肝癌细胞生长抑制作用；使用Annexin V-FITC／PI双染色，经流式细胞仪检测鸢尾异黄酮对细胞早期凋亡率的影响。结果：鸢尾异黄酮明显减少肝癌细胞贴壁数量，使癌细胞变小变圆；在1．0～8．0μg·mL^-1范围内浓度依赖性地抑制人肝癌细胞SMMC-7721的增殖；鸢尾异黄酮在5．00，10．00和20．00μg·mL^-1浓度下均能显著提高肝癌细胞株早期凋亡率（P〈0．01）。结论：鸢尾异黄酮对人肝癌细胞SMMC-7721增殖具有明显的抑制作用，其机制可能与促进癌细胞凋亡有关。Objective： To evaluate inhibitory effect of tectorigenin from Pueraria flos on proliferation of human hepatocarcinoma SMMC-7721 cells. Methods： Tectorigenin was added into the cultures of SMMC-7721 cells in log phase growth. The morphologic changes were observed 24 h after treatment with an inverted microscope. The inhibitory effect on proliferation of tumor cells was evaluated by MTT assay. The rate of early apoptosis was measured by flow eytometer using Annexin V-FITC/PI double staining. Results： After tectorigenin treatment for 24 h, the cells became smaller, rounder and thicker. The adherent cells were much fewer after tectorigenin treatment. MTT assay showed that tectorigenin （ 1.0 - 8.0 μg·mL^-1 ） inhibited cell proliferation in a concentration-dependence manner. The early apoptosis rate after treatment with 5.00, 10.00 and 20.00μg·mL^-1 teetorigenin were significantly higher than control（P 〈 0.01 ）. Conclusion： Tectorigenin has significantly inhibitory effect on proliferation of SMMC-7721 cells, which is related to promoting apoptosis.

关 键 词：鸢尾异黄酮 细胞凋亡 抗肿瘤活性 ANNEXIN V/PI双染色法 

分 类 号：R96[医药卫生—药理学] R979.1[医药卫生—药学]