嗜酸乳杆菌与白色假丝酵母菌交互作用  

Interaction Between Candida albicans and Lactobacillus acidophilus

作　　者：李桂军[1] 蒋琰瑛[2] 张红霞[2] 周建娟 

机构地区：[1]嘉兴市妇幼保健院检验科生殖医学中心,浙江嘉兴314000 [2]嘉兴市妇幼保健院生殖医学中心,浙江嘉兴314000 [3]嘉兴市妇幼保健院急诊科,浙江嘉兴314000

出　　处：《中华医院感染学杂志》2010年第3期316-319,共4页Chinese Journal of Nosocomiology

基　　金：嘉兴市科技局计划项目(2008AY2035-2)

摘　　要：目的对嗜酸乳杆菌与白色假丝酵母菌间的交互作用及pH、乳酸、乙酸、H2O2等对白色假丝酵母菌生长影响进行评价，为细菌性阴道病治疗后易转为外阴阴道假丝酵母菌病的原因提供依据。方法不同浓度嗜酸乳杆菌、白色假丝酵母菌混合培养；白色假丝酵母菌经不同pH值培养基培养、1％乳酸、I％乙酸、3％H2O2作用，检测其浓度变化，形态学观察白色假丝酵母菌形态变化。结果嗜酸乳杆菌浓度依赖性抑制白色假丝酵母菌生长，白色假丝酵母菌可促进嗜酸乳杆菌生长；pH值为3．5～5．5时，嗜酸乳杆菌具有较强的抑菌能力，当pH值为4．0时，嗜酸乳杆菌抑菌能力最强；乳酸、乙酸均对白色假丝酵母菌生长有明显抑制作用且具有浓度和时间依赖性，Hzoz对白色假丝酵母菌生长并无直接抑制作用。结论嗜酸乳杆菌主要通过其代谢产物乳酸而不是H2O2直接产生抑菌作用，细菌性阴道病治疗后转为外阴阴道假丝酵母菌病的发生，主要与乳杆菌因某些因素生长受抑，导致数量降低及其产酸能力下降有关。OBJECTIVE To evaluate the interaction between Candida albicans and Lactobacillus acidophilus and to study the effect of pH, lactic acid, acetic acid and hydrogen peroxide on C. albicans. METHODS The interaction between C. albicans and L. acidophilus was evaluated using MRS liquid culture method. The concentration change of C. albicans was observed after treated with pH, 1% lactic acid, 1% acetic acid and 3% hydrogen peroxide. RESULTS L. acidophilus, lactic acid, acetic acid but hydrogen peroxide inhibited C. albicans growth in dose and time dependent manner. L. acidophilus had stronger hacteriostasis when pH was 4. 0, C. albicans promoted L. acidophilus growth too. CONCLUSIONS L. acidophilus executes bacteriostatic action by lactic acid not hydrogen peroxide. Vulvovaginal candidiasis is correlated with Lactobacillus quantity and their acidic production

关 键 词：细菌性阴道病 外阴阴道假丝酵母菌病 嗜酸乳杆菌 白色假丝酵母菌 

分 类 号：R379[医药卫生—病原生物学]