牛乳中超氧化物歧化酶(SOD)的提纯与鉴定  

PURIFICATION AND IDENTIFICATION OF SUPEROXIDE DISMVTASE FROM MILK

作　　者：孙庆林[1,2,3] 常梅秀 周书勤 姜广军 

机构地区：[1]内蒙古农牧学院生物工程系 [2]包头市第六中学 [3]内蒙古农牧学院食品工程系

出　　处：《内蒙古农牧学院学报》1998年第4期34-36,共3页

摘　　要：新鲜牛乳经超速离心，氯仿乙醇处理，丙酮沉淀、透析、ＤＥＡＥ纤维素柱层析等步骤使其中Ｃｕ－ＺｎＳＯＤ得到了纯化，提纯后酶的比活为１４９．５Ｖ／ｍｇ。该酶对Ｈ２Ｏ２敏感，并且在２７２ｎｍ和６７５ｎｍ处各有一吸收峰，证明被纯化的酶是Ｃｕ－ＺｎＳＯＤ。A copper/zinc superoxide dismutase from fresh milk was purified to homogeneity by ultracentrifugztion. Chloroform ethanol treatment. acetone sedimention, dialyzation, DEAE-cellulose chromatography. the specific activity of the purified enzyme is 149.5V/mg. The enzyme is sensitire to H 2 O 2 .The enzyme enhibits maximam absortion at 272nm and 675nm. This facts have proved that the enzyme is copper/zinc SOD.

关 键 词：牛乳 超氧化物歧化酶 纯化 鉴定 

分 类 号：TS252.2[轻工技术与工程—农产品加工及贮藏工程] Q550.3[轻工技术与工程—食品科学与工程]