红谷霉素的抗细菌活性和毒力测定  

The Study on the Antibacterial Ability and Virulence Intensity of Honggumycin to Bacterium

作　　者：敖康[1] 涂晓嵘[1] 涂璇[1] 张晓阳[1] 涂国全[1] 

机构地区：[1]江西农业大学生物科学与工程学院,南昌330045

出　　处：《中国农学通报》2010年第2期55-59,共5页Chinese Agricultural Science Bulletin

基　　金：江西省自然科学基金资助项目"链霉菌702所产抗细菌物质S2抗菌机制的研究"0630013)

摘　　要：为了探索红谷霉素的抗细菌活性和毒力方程。分别以红谷霉素为试验材料9种细菌为供试菌株,通过比浊法测定红谷霉素的抑菌能力和红谷霉素对各种供试细菌的抑菌能力的大小。试验结果表明,在所选用的9种供试细菌中,红谷霉素对其中8种供试细菌的EC50和EC90依次为:金黄色葡萄球菌,0.002 mg/L和0.017 mg/L、腊质芽孢杆菌,0.02 mg/L和0.19 mg/L、巨大芽孢杆菌,0.08 mg/L和0.44 mg/L、痢疾杆菌,0.17 mg/L和1.87 mg/L、青枯假单孢菌,0.26 mg/L和2.3 mg/L、大肠杆菌,0.73 mg/L和4.49 mg/L、柑桔溃疡病菌,0.73 mg/L和5.0 mg/L、水稻白叶枯病菌,124 mg/L和226 mg/L。红谷霉素分别对其余8种供试细菌都有很强的抑制作用。The purpose of this study was to grope for antibacturial ability and virulence intensity of Honggumycin.Honggumycin was used as experimental material and nine kinds of bacterium was used as the tested strains separately. The anti bacterium and virulence intensity of Honggumycin to bacterium is tested by the method of turbidity comparing. In all the nine tested bacterium, Honggumycin has anti bacterium for the eight bacterium except for Aeromonas hydropila. EC50 and EC90 of the eight bacterium were as follows： Staphylococcus aureus, 0.002mg/L and 0.017mg/L, Bacillus cereus, 0.02 mg/L and 0.19 mg/L, Bacillus megaterium, 0.08 mg/L and 0.44 mg/L, Sh.flexneri, 0.17 mg/L and 1.87 mg/L, Pseudomonas solanaeearum, 0.26 mg/L and 2.3 mg/L, Escherichia coli,0.73 mg/L and 4.49 mg/L, Xanthomonas citri, 0.73 mg/L and 5.0 mg/L, Xanthomonas oryzae, 124 mg/L and 226 mg/L. Honggumycin have a very strong inhibition to eight of the rest kinds of the tested bacteria respectively.

关 键 词：链霉菌702 红谷霉素 抑菌活性 毒力方程 

分 类 号：S3[农业科学—农艺学]