三种标志物对监测肺癌患者血液微转移的评价  

An estimate on three markers in the monitoring of blood metastasis of lung cancer

作　　者：卢冬梅[1] 邸志强[3] 薛承岩[1] 杜新生[1] 杨林瀛[2] 

机构地区：[1]承德医学院附属医院检验科,067000 [2]承德医学院附属医院呼吸科,067000 [3]承德钢铁集团有限公司职工医院内科

出　　处：《肿瘤研究与临床》2010年第1期41-43,共3页Cancer Research and Clinic

摘　　要：目的了解检测外周血液癌胚抗原mRNA（CEAmRNA）、细胞角蛋白19mRNA（CK19mRNA）和端粒酶在监测肺癌血液转移中的应用价值。方法外周血液标本应用反转录-套式-聚合酶链反应检测103例肺癌患者（肺癌组）、57例非癌性肺部疾病患者（非肿瘤组）及36例健康志愿者（健康组）CEAznRNA和CK19mRNA，端粒重复序列扩增-微孔板杂交-酶联免疫分析检测端粒酶。结果肺癌组血液三种肿瘤标志物的阳性率显著高于非肿瘤组和健康组（P〈0．001），CEAmRNA和端粒酶的敏感度显著高于CKl9mRNA（P〈0．01），TNMⅢ、Ⅳ期的阳性率显著高于Ⅰ期（P〈0．05）。结论检测血液CEAmRNA、CK19mRNA和端粒酶有助于发现肺癌血液扩散，其中CEAmRNA和端粒酶的价值高于CK19mRNA，联合检测可进一步提高敏感度。Objective To understand the clinical value on the detection of carcinoembryonie antigen mRNA （CEA mRNA）, eytokeratin 19 mRNA （CK19 mRNA） and telomerase in blood for the monitoring of blood metastasis of lung cancer. Methods CEA mRNA and CK19 mRNA were detected by reverse transeriptase-nested primers-polymerase chain reaction, telomerase was detected by telomeric repeat amplification protocol-hybridism-enzyme-linked immunosorbent assay in peripheral blood. Results The positive rates of the three tumor markers in lung cancer group were much higher than the non-tumor group and the health group （P 〈0.001）. The sensibility of CEA mRNA and telomerase were much higher than CK19 mRNA （P 〈0.01）. The positive rates of the markers in TNM stages DI and Ⅳwere much higher than in stages Ⅰ （P 〈0.05 to P 〈0.01）. Conclusion It had high value that detecting CEA mRNA, CK19 mRNA and telomerase in peripheral blood on the discovery of blood metastasis of lung cancer. Among them, the clinical value of CEA mRNA and telomerase are higher than CK19 mRNA, and combined assay of the markers can improve the sensibility.

关 键 词：肺肿瘤 肿瘤转移 肿瘤标记 生物学 

分 类 号：R734.2[医药卫生—肿瘤]