克氏原螯虾(Procambarus clarkii)应急二价铜离子时超氧化物歧化酶和过氧化氢酶的变化  

Activity Change of Superoxide Dismutase and Catalase of Crayfish (Procambarus clarkii) under Copper Ion Stress

作　　者：李忠[1] 梁宏伟[1] 呼光富[1,2] 王长忠[1,2] 吴勤超[1,2] 罗相忠[1] 邹桂伟[1] 

机构地区：[1]中国水产科学研究院长江水产研究所,湖北荆州434000 [2]华中农业大学水产学院,湖北武汉430070

出　　处：《武汉大学学报（理学版）》2010年第1期115-119,共5页Journal of Wuhan University:Natural Science Edition

基　　金：国家科技基础条件平台专项(2006DKA30470-002);中国水产科学院长江水产研究所所长基金资助项目

摘　　要：用二价铜离子(Cu2+)胁迫克氏原螯虾,分析其肝胰腺、触角腺、鳃等组织的超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活性变化.结果表明:克氏原螯虾能在含10mg/LCu2+水中生活;在10mg/LCu2+胁迫下,在0～24h,肝胰腺和触角腺中的CAT、SOD活性均显著升高,CAT活性在24h达到最高;在24～36h,肝胰腺和触角腺中的CAT活性开始下降,而SOD活性继续上升,在第36h时达到最高;48h后,肝胰腺和触角腺中SOD和SOD活性下降并均趋于平稳表达,SOD活性仍显著高于非胁迫处理时,而CAT活性则显著低于Cu2+处理时;鳃中的SOD和SOD活性未受到Cu2+浓度变化的影响.Crayfish, （Procambarus clarkii） was treated with different concentrations of Cu^2＋ , the activity of superoxide dismutase （SOD） and catalase （CAT） in hepatopancreas, antennary gland and gill measured. It was found that crayfish survived 10 mg/L Cu^2＋ , in which the activity of SOD and CAT in hepatopanereas and antennary gland increased during 0-24 hour and the activity of CAT reach its max at 24 hour; after 24 hour, CAT in hepatopancreas and antennary gland gradually decreased, SOD still increased and reach it max level at 36th hour; after 48 hour SOD and CAT of hepatopancreas and antennary gland were decreased and reach a stable level, SOD activity were increase and CAT activity was decreased significantly compare with controlled; while the activity of SOD and CAT in gill showed no evident changes during the process of Cu^2＋ treatment.

关 键 词：克氏原螯虾 二价铜离子 超氧化物歧化酶 过氧化氢酶 

分 类 号：Q175[生物学—水生生物学] X503.225[生物学—普通生物学]