黄芪甲苷、β-榄香烯对SGC7901胃癌细胞COX-2及PGE_2表达的影响  

Effect of Astragaloside and β-elemene on expression of COX-2 and PGE_2 in gastric cancer cell line SGC7901

作　　者：曹丽萍[1] 沈洪[2] 刘丽[2] 刘云[3] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210029 [2]江苏省中医院,江苏南京210029 [3]南京医科大学第一附属医院,江苏南京210029

出　　处：《现代中西医结合杂志》2010年第7期798-800,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：江苏省自然科学基金资助项目(BK2008492)

摘　　要：目的观察黄芪甲苷、β-榄香烯对人胃癌细胞中COX-2、VEGF和PGE2表达的影响。方法采用MTT试验观察黄芪甲苷、β-榄香烯对胃癌细胞的抑制作用,用RT-PCR以及Western blot方法研究加药后人胃癌细胞C0X-2基因及其蛋白表达的变化,用ELISA方法检测培养基中VEGF、PGE2的表达情况。结果黄芪甲苷、β-榄香烯能抑制人胃癌细胞生长,呈一定的量效特征;并能抑制人胃癌细胞COX-2、VEGF和PGE2的表达。结论黄芪甲苷、β-榄香烯抗肿瘤的机制可能是通过抑制COX-2,进而抑制其下游产物PGE2的表达及使VEGF表达下调,从而抑制肿瘤的生长。Objective It is to observe the effect of Astragaloside and β - elemene on the expression of COX - 2, vascular endothelial growth factor（VEGF） and prostaglandin E2 （PGE2 ） in human gastric cancer cell. Methods The inhibitory effect of Astragaloside and β- elemene on viability of SGC7901 cell was assessed by MTT assay. The expression of COX 7 2 mRNA and protein was determined by RT- PCR and Western blot respectively. The expression of VEGF and PGE2 in culture medium was detected by ELISA. Results Astragaloside and β- elemene inhibited growth of SGC7901 cell in a dose-dependent manner and down-regulated expression of COX - 2,VEGF and PGE2. Conclusion The anti-tumor mechanism of Astragaloside and β - elemene is related with inhibition of COX - 2 expression, which causes the suppression of its downstream product PGF2 expression and down-regulation of VEGF, therefore decreases tumor growth.

关 键 词：黄芪甲苷 Β-榄香烯 胃癌 环氧合酶 血管内皮生长因子 前列腺素E2 

分 类 号：R-332[医药卫生]