外伤性癫痫灶中泛素蛋白酶体系的形态学改变  

Morphology Changes of Ubiquitin-proteasome System in Traumatic Epilepsy

作　　者：何鹏翔[1,2] 唐运林[3] 蔡卫林[1] 黄永华[1] 方超[1] 竞花兰[1] 

机构地区：[1]中山大学中山医学院法医系,广东广州510080 [2]上海市公安局金山分局,上海200540 [3]中国人民解放军广州空军医院,广东广州510602

出　　处：《法医学杂志》2010年第1期10-14,共5页Journal of Forensic Medicine

摘　　要：目的检测泛素(ubiquitin,Ub)和泛素激活酶(ubiquitin-activating enzyme1,UbE1)在外伤性癫痫灶中的表达,探索其对鉴定外伤性癫痫的应用价值。方法收集脑外伤后癫痫灶皮质样本15例(外伤性癫痫组)、非脑外伤后癫痫灶皮质样本15例(非外伤性癫痫组),交通事故死亡正常脑皮质样本15例(非癫痫组),通过HE常规染色观察各组形态学改变,用免疫组化染色检测3组中Ub和UbE1的表达情况。结果常规HE染色下,与非癫痫组相比,外伤性癫痫组和非外伤性癫痫组出现神经元数量减少、神经元变性等形态学改变,外伤性癫痫改变更明显。Ub和UbE1主要表达在癫痫灶神经元细胞核、细胞质内,细胞外未见表达。图像分析显示两指标的表达量为外伤性癫痫组>非外伤性癫痫组>非癫痫组,差别具有统计学意义(P<0.05)。结论正常神经元数量减少、神经元变性是癫痫灶的主要病理改变之一,外伤性癫痫神经元病理改变更明显。与HE染色比较,Ub和UbE1免疫组化染色更灵敏,有助于鉴别外伤性癫痫和非外伤性癫痫。Objective To investigate the value of ubiquitin（Ub） and ubiquitin-activating enzymel （UbE1） for the appraisement of post traumatic epilepsy （PTE）. Methods Fifteen specimens from human epileptic temporal cortex originating from PTE were collected as the PTE group. Fifteen specimens from non-PTE were collected as the non-PTE group. Meanwhile, 15 normal cerebral cortex specimens from people dead from acute traffic accident were collected as the control groups. Observe morphology changes of each group with HE, then with immunohistochemistry of Ub and UbE1. Results Compared to the control group, morphology changes of neuron quantity reduction, neuron denaturation and so on were observed both in the PTE group and the non-PTE group under HE, especially in the PTE group. Ub and UbE1mainly expressed in the nucleus and cytoplasm of the neurons in epilepsy spot without extracellular expression. The expression of Ub and UbE1 is PTE group 〉 non-PTE group 〉 control group （P〈0.05）. Conclusion The neuron denaturation are one of the main pathology changes of epilepsy, and it is more obvious in the PTE group. Immunohistochemistry of Ub and UbE1 may be more helpful to distinguish PTE and non-PTE than HE staining.

关 键 词：法医病理学 癫痫 创伤后 泛素 泛素激活酶类 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R737.3[医药卫生—临床医学]