结缔组织生长因子对正畸大鼠牙周组织改建的影响  

Effects of CTGF on the periodontal tissue remolding during orthodontic tooth movement

作　　者：胡江天[1] 李松[2] 高国杰[1] 余兵[1] 沈绍莹[1] 刘亚丽[1] 

机构地区：[1]昆明医学院附属口腔医院正畸科,650031 [2]昆明医学院

出　　处：《实用口腔医学杂志》2010年第1期24-28,共5页Journal of Practical Stomatology

基　　金：云南省科技厅-昆明医学院联合专项基金(编号:2007C0028R);云南省教育厅科学研究基金项目(编号:07Y10706)

摘　　要：目的:探讨结缔组织生长因子(connective tissue growth factor,CTGF)对大鼠正畸牙移动模型牙周组织改建的影响。方法:雄性SD大鼠45只,牵引下颌第一磨牙近中移动制作大鼠牙移动模型。模型完成后,随机分为3个实验组:CTGF组,生理盐水对照组及加力对照组,每组15只。从0 d开始分别将CTGF及生理盐水每隔1 d注射到CTGF组及生理盐水对照组左侧下第一磨牙舌侧骨黏膜下,分别于干预后1、3、7、15、21 d每组各取3只大鼠的牙周组织,制备组织切片,HE及免疫组化染色,计算机图像分析检测牙周组织中VEGF的表达状况。结果:正畸加力后,大鼠牙周组织中伴随牙周组织改建,牙周组织中VEGF表达增高。加力7 d牙周组织改建最为活跃,VEGF表达达到高峰期。CTGF干预治疗后,CTGF组加力后7、15 d VEGF表达强度增幅最高,CTGF组与对照组间差异有显著性。结论:CTGF干预可上调正畸大鼠牙周组织中VEGF表达,加速正畸牙周组织改建。Objective：To study the effects of CTGF on the periodontal tissue remolding during orthodontic tooth movement.Methods：A mouse model of orthodontic tooth movement was established.The CTGF and NS were injected into the periodontal tissue of the first molar.HE staining,immunohistochemical staining and automatic image analysis system were used to detect the changes of periodontal tissue and the expression of VEGF.Results：The periodontal tissue remolding accompanied the over expression of VEGF during the orthodontic tooth movement.At day 7,the remolding was most active and the expression of VEGF was the highest.CTGF injection induced acceleration of periodontal remolding and up-regulating the expression of VEGF during orthodontic tooth movement,especially at day 7 and day 15 after the orthodontic force were applied.Conclusion：In the mouse model of orthodontic tooth movement,periodontal CTGF injection induces over expression of VEGF and accelerates the periodontal remodeling during orthodontic tooth movement.

关 键 词：结缔组织生长因子 正畸治疗 牙移动 大鼠 

分 类 号：R783.5[医药卫生—口腔医学]