不同扩张方式对扩张皮肤胶原纤维和肌动蛋白的影响  

Effects of different skin expansion ways on collagen and actin

作　　者：施文斌[1] 董玉英[1] 董福生[1] 石培凯[1] 任贵云[1] 李建英[1] 

机构地区：[1]河北医科大学口腔医学院口腔颌面外科,石家庄050017

出　　处：《实用口腔医学杂志》2010年第1期38-42,共5页Journal of Practical Stomatology

基　　金：河北省科学技术研究与发展计划项目(编号:08276101D-59)

摘　　要：目的:探讨不同扩张方式对皮肤胶原纤维和肌动蛋白的影响,为临床上寻找合适的扩张方式提供实验依据。方法:选用随机分组法,将实验兔分为反复快速扩张组(A组)、快速扩张组(B组)、常规扩张组(C组)、埋入不扩张组(D组)、正常对照组(E组)。实验组埋植扩张器并进行不同方法的扩张,不同的维持期取材。应用特殊染色法检测皮肤中Ⅰ、Ⅲ型胶原纤维的含量及其比值;应用免疫组化检测肌动蛋白的表达。结果:A、B、C组早期以I型胶原纤维为主且较粗大,排列较为紊乱,Ⅲ型胶原纤维相对较少。4周后,I型胶原纤维排列逐渐规律,Ⅲ胶原纤维相对增加。Ⅰ、Ⅲ型胶原面积比较:相同维持期A、B、C组间差异无显著性;A、B 2组随着维持期的延长呈增长趋势,4周后趋于稳定。C组随着维持期的延长呈增长趋势,3周后趋于稳定。Ⅰ/Ⅲ型胶原比值比较:A、B 2组在前3周时,与E组相比差异有显著性,在4周后差异无显著性;C组在前2周时差异有显著性,在3周后差异无显著性。A、B、C 3组内肌动蛋白阳性率随着维持期的延长呈下降趋势。相同维持期A、B、C 3组间比较:B组>A组>C组,经统计学分析,前3周时,3组间差异均有显著性;在4周时A、C 2组间差异已无显著性,但在4～6周时A、B 2组间差异仍有显著性。结论:反复快速扩张可缩短疗程,并能获得高质量的皮肤,是一种安全可行的扩张方法。Objective： To understand the effects of different expansion ways on collagen and actin of skin and to provide an experiment foundation for the suitable expansion method clinically.Methods： The rabbits were divided into five groups.The expanders were embedded in experimental group.The sirius-red specific staining method was applied to observe the changes of type I collagen and type III collagen and their proportion.Immunochemistry method was used to detect the expression of actin.Results： 1： In group A,B and C,type I collagen arranged turbulently and was thicker and type III collagen decreased relatively at earlier maintaining period.After 4 week,type I collagen arranged regularly and type III collagen was relatively increased.2： Comparisons of the area about type I,III collagen： At the same maintaining period,there were no significant difference among the group A,B and C.With the extension of maintaining period,the area of type I,III collagen increased gradually,but there was inclined to stabilize at the fourth week in group A,B and at the third week in group C.Comparison of the proportion of collagen I and collagen III： There were significant difference between group A,B and group D,but no significant difference was observed after the fourth week.There were significant difference between group C and control group,but no significant difference was observed after three week.3.With the extension of maintaining period,the positive expression rates of actin decreased gradually in group A,B,C.At the same maintaining period,significant difference were observed among group A,B,C.The positive expression rates of actin in group C were lower than that of group A at the same maintaining period.Conclusion： The repeated rapid expansion can not only shorten the clinical period but also promote the quality of skin flap.It is a safe and feasible method in clinic.

关 键 词：皮肤扩张 胶原纤维 肌动蛋白 免疫组化 苦味酸天狼猩红染色 

分 类 号：R78[医药卫生—口腔医学]