LL37多肽及其mRNA在口腔扁平苔藓病损表达研究  

The expressions of LL37 and LL37 mRNA in OLP lesions

作　　者：陶人川[1] 邹杰[1] 杨亦萍[2] 曾启新[1] 

机构地区：[1]广西医科大学口腔医院牙周黏膜科,南宁530021 [2]广西医科大学口腔医院口腔病理科,南宁530021

出　　处：《实用口腔医学杂志》2010年第1期60-63,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金项目(编号:30860313);广西回国基金项目(编号:桂科回0639019)

摘　　要：目的:探讨LL37在口腔扁平苔藓(oral lichen planus,OLP)病损中的表达及在OLP疾病机制中的作用。方法:采用免疫组织化学技术(IHC)和逆转录聚合酶链式技术(RT-PCR)检测25例OLP患者的病变黏膜组织和7例健康对照黏膜组织中LL37蛋白及其mRNA的表达。结果:IHC结果显示,LL37在所有样本中均呈阳性胞质染色,主要表达于棘层和淋巴细胞。25例OLP组口腔病损黏膜均呈强阳性染色,较之健康口腔黏膜组中LL37为弱阳性染色,差异具有显著性(P<0.05);RT-PCR检测结果显示,所有样本均检测出LL37的cDNA电泳条带,健康口腔黏膜组中仅呈微弱表达,而OLP组病损黏膜上皮LL37表达明显增强。结论:LL37可能因炎性诱导呈上调表达而参与OLP的发生发展过程。Objective： To investigate the expression of LL37 in oral lichen planus（OLP） lesions.Methods： 25 OLP and 7 health controls were included in this study.IHC and RT-PCR methods were used to detect the LL37 expression on protein level and mRNA level.T-test was used for data analysis.Results： Positive staining of LL37 was found in all the samples in cytoplasm of epithelium cells using IHC method,and mostly in prickle and basal layers.But the reaction intensity in health control was weak,compared to the strong signal in OLP group（P0.05）.The expressions of LL37 mRNA in OLP were significantly higher than those in health controls（P0.05）.Conclusion： The expression of LL37 on normal oral mucosa suggests that it may play a role in maintaining the health of oral mucosa as a part of innate immunity.The up-regulation of LL37 under inflammation status might participate in the immunopathological development of OLP.

关 键 词：口腔扁平苔藓 LL37 先天性免疫 

分 类 号：R781.5[医药卫生—口腔医学]