脾脏切除对颅脑创伤大鼠死亡率及脑含水量的影响  

Effect of splenectomy on mortality and brain water content of rats with brain injury

作　　者：李梅[1] 李飞[1] 张礼均[1] 陈志[1] 张项[1] 单佑安[1] 林江凯[1] 朱刚[1] 尹志勇[2] 冯华[1] 

机构地区：[1]第三军医大学附属西南医院神经外科,全军神经系统疾病微创诊治专科中心,重庆400038 [2]第三军医大学附属大坪医院野战外科研究所四室

出　　处：《中华创伤杂志》2010年第1期9-12,共4页Chinese Journal of Trauma

基　　金：卫生部行业公益性基金资助项目（200802017）;国家重点基础研究发展规划前期专项资助项目（2007CB516704）

摘　　要：目的观察脾脏切除对脑创伤大鼠死亡率及脑组织含水量的影响，为提高重型颅脑创伤患者的救治水平探讨新思路。方法成年雄性SD大鼠采用随机数字表法分为三组：颅脑创伤假手术＋脾切除假手术组（A组），颅脑创伤＋脾切除假手术组（B组）和颅脑创伤＋脾切除组（C组）。采用改进的Feeney法对大鼠右侧大脑半球进行致伤，伤后采用Longa法进行行为学评分；观察A（23只）、B（48只）、C（47只）三组大鼠致伤后7d内的死亡率；测定各组致伤后1d（8只）、2d（8只）、3d（8只）及7d（7只）时各组大鼠脑组织含水量。结果致伤后B、C两组之间Longa评分差异无统计学意义（P〉0．05）；致伤后7d内大鼠死亡率A、B、C组分别为O％、35．4％及14．9％，各组间比较差异均有统计学意义（P〈0．05）；B、C组致伤侧脑含水量于伤后1，2，3及7d时分别为（81．98±0．35）％和（81．78％±0．41）％、（82．58±0．63）％和（81．81±0．48％）（P〈0．05）、（82．54±0．54）％和（81．52％±0．84）％（P〈0．05），以及（81．50±0．41）％和（81．21±0．36）％。结论颅脑创伤大鼠于伤后行脾脏切除能够降低致伤侧脑组织水肿程度，显著降低大鼠的死亡率。Objective To observe the effect of splenectomy on mortality and brain water content of rats with brain injury so as to explore novel way for better clinical management of patients with severe traumatic brain injury. Methods Adult male SD rats were randomly divided into three groups, ie, sham operation on brain and spleen （ Group A, n = 23 ）, experimental brain trauma ＆ sham operation on spleen （ Group B, n ： 48） and experimental brain injury ＆ splenectomy （ Group C, n = 47 ）. Modified Feeney＇ s method was used to create the animal model of experimental brain trauma, Longa＇ s scale was applied to evaluate the neurologic defect. Mortality within seven days following brain injury was calculated. In the meantime, the brain water content was detected at days 1 （ n = 8 ）, 2 （ n = 8 ）, 3 （ n = 8 ） and 7 （ n = 7） after brain injury in each group, Results No statistical difference of Longa＇ s scale was found between Group B and Group C （P 〉 0.05 ）. The mortalities within seven days after brain injury were 0%, 35.42 and 14.89% in Groups A, B and C respectively, with statistical difference between groups （P 〈0.05）. The brain water content of Groups B and C at days 1, 2, 3 and 7 were （81.98 ± 0.35）% ＆ （81.78 ±0.41）%, （82.58 ±0.63）% ＆ （81.81 ±0.48）% （P〈0.05）,（82.54 ± 0.54）% ＆ （81.52±0.84）% （P〈0.05） and （81.50±0.41）% ＆ （81.21 ±0.36）% （P〉0.05） respectively. Conclusion Splenectomy can effectively reduce brain water content and significantly decrease mortality in rats with brain injury.

关 键 词：脑损伤 脾切除术 死亡率 脑含水量 

分 类 号：R651.15[医药卫生—外科学] R657.6[医药卫生—临床医学]