Claudin-5和claudin-10在结直肠癌中的表达及其意义  

Ciaudin-5 and claudin-10 expression in colorectal carcinoma

作　　者：裴海平[1] 蒋锐[1] 葛华[1] 曹慧[1] 

机构地区：[1]中南大学湘雅医院胃肠外科,长沙410008

出　　处：《中华普通外科杂志》2010年第1期40-43,共4页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目（30872463）;湖南省自然科学基金资助项目（07JJ5016）

摘　　要：目的研究claudin-5和claudin-10在结直肠癌中的表达及其意义。方法应用免疫组化sP法（streptavidin perdcidase）检测经病理证实的50例正常结直肠黏膜（正常组），25例结直肠腺瘤（腺瘤组），25例无淋巴结转移及25例伴淋巴结转移的结直肠癌（癌组）中claudin-5和claudin-10的表达。统计学方法采用X^2检验。结果claudin-5阳性表达率在正常组，腺瘤组，癌组分别为82％，76％，68％，方差分析提示多组间差异无统计学意义（X^2=2．638，P〉0．05）。claudin-5的表达与淋巴结转移有关（P〈0．05），号性别，年龄，肿瘤部位，分化程度，肿瘤直径，浸润深度无关（P〉0．05）。claudin-10阳性表达率在正常组，腺瘤组，癌组分别为54％，56％，72％，方差分析提示多组间差异无统计学意义（x。=3．839，P〉0．05）。claudin-10的表达与肿瘤直径，浸润深度，淋巴结转移有关（P〈0．05），与性别，年龄，肿瘤部位，分化程度无关（P〉0．05）。结直肠癌中claudin-5与claudin-10的表达无相关性（r=0．050，P：0．732）。结论claudin-5和claudin-10在正常结直肠黏膜，结直肠腺瘤，结直肠癌中均有表达，它们与结直肠癌的发生无关。claudin-5和claudin-10均与结直肠癌淋巴结转移相关，claudin-10还与肿瘤直径，浸润深度相关。结直肠癌中claudin-5与claudin-10的表达无明显相关。Objective To investigate the expressions of claudin-5 and claudin-10 in colorectal carcinoma（ CRC ） and its significance. Methods Pathological verified 50 colorectal tissue （CRT）, 25 coloreetal adenoma （CRA）, 25 non lymph node metastasis CRC （non-LNM CRC ） and 25 lymph node metastasis CRC （LNM CRC ） were detected for the expression of claudin-5 and claudin-lO by immuno- histochemical SP（ streptavidin perdcidase） method. Results The positive expression rate of Claudin-5 was 82% ,76% ,68% in CRT, CRA, CRC, respectively. The positive expression rate of claudin-5 in different groups was not significantly different（ X^2 = 2. 638 ,P 〉 0. 05 ）. claudin-5 expression was correlated with LNM （P 〈 0. 05 ）, but was not correlated with gender, age, tumor, location, differentiation, tumor diameter and serous membrane invasion（P 〉 0.05）. The positive expression rate of claudin-10 was 54% ,56% ,72% in CRT, CRA, CRC, respectively. The positive expression rate of claudin-10 in different groups was not significantly different（ X^2 = 3. 839,P 〉 0. 05 ）. claudin-10 expression was correlated with tumor diameter, serous membrane invasion and LNM （ P 〈 0.05 ）, but was not correlated with gender, age, location and differentiation （ P 〉 0. 05 ）. There was no significant correlation between claudin-5 expression and claudin-10 expression in CRC （r = 0. 050, P = 0. 732）. Conclusions claudin-5 and claudin-10 are expressed in CRT, CRA, and CRC. They are not involved in CRC occurrence, claudin-5 and claudin-10 abnormally expressions are significantly associated with the incidence of LNM. Meanwhile, claudin-10 expression is correlated with tumor diameter and serous membrane invasion. There was no significant correlation between claudin-5 expression and claudin-10 expression in CRC.

关 键 词：结直肠肿瘤 免疫测定 连接蛋白类 

分 类 号：R735.3[医药卫生—肿瘤] R739.41[医药卫生—临床医学]