小鼠Lewis肺癌原位模型的构建  

Establishment of Orthotopic Lewis Lung Cancer Model in Mouse

作　　者：刘馨[1] 伍治平[1] 左曙光[1] 周永春[1] 陈艳[1] 王熙才[1] 

机构地区：[1]昆明医学院第三附属医院(云南省肿瘤医院)肿瘤研究所,昆明650118

出　　处：《中国肺癌杂志》2010年第1期42-47,共6页Chinese Journal of Lung Cancer

基　　金：云南省联合专项基金(No.2007C0024R)资助~~

摘　　要：背景与目的肺癌原位模型包括小鼠自发性肺肿瘤模型和气道内接种模型等,但自发性肺肿瘤模型耗时较长且成瘤率不能保证,而气道内接种模型成瘤部位及大小不稳定。本研究以3LL细胞系细胞接种于C57BL/6小鼠肺原位,与皮下接种模型比较,探讨其稳定性、转移特性,并建立小鼠肺原位癌模型的优化方法。方法将不同数量级的3LL细胞分别直接接种于C57BL/6小鼠左侧腋下制备皮下接种模型和以Matrigel悬浮后接种于其左肺制备原位接种模型,观察两种模型的生存期,并对小鼠进行解剖后行病理切片检查、免疫组化染色检测微血管密度、流式细胞仪检测CD44v。结果皮下组成瘤率分别为100%、66.7%、16.7%,未见明显转移。原位组成瘤率分别为100%、100%、83.3%,并可转移至对侧胸廓及肺脏。原位组中位生存期(38d、35d、23d)明显少于皮下接种组(82d、72d、50d)。原位接种组微血管密度(120.2±9.73)高于皮下组(92.6±7.12)。原位接种组肿瘤细胞悬液CD44v表达(26.46±1.56)%高于皮下接种组(23.13±1.02)%。结论以3LL细胞接种于小鼠肺部所建立的肺癌原位模型简单可靠,重复性高,具有较皮下接种模型更强的转移特性。Background and objective The mouse lung cancer orthotopic model includes spontaneous lung cancer model and endotracheal transplanted model, and etc. The spontaneous lung cancer needs longer time and does not ensure the rate of the generation of the tumor; as for endotracheal transplanted model, the position and size of the tumor are instable. In this study, the 3LL cell line was orthotopically transplanted into the lung of the CS7BL/6 mice, compare to the heterotopic model, to discuss their stability and transfer-characteristics. And this study was also to optimize the method of establishing lung cancer orthotopic animal model. Methods Different quantity of 3LL cells were inoculated into the left oxter of CS7BL/6 mice to establish the heterotopic model; or suspended with Matrigel then inoculated into the left lung of CS7BL/6 mice to establish orthotopic model. The survival-time of the mice was examined. The tissue was collected for the subsequent histology assay after euthanizing the mice. Microvessels density （MVD） was observed and counted by immunohistological chemistry. CD44v was detected byflow cytometry. Results TTumor-form-rate ofthe heterotopic group were 100%, 66.7%, 16.7%, respectively, and had no macroscopic transfer. Tumor-form-rate of the orthotopic group were 100%, 100%, 83.3%, respectively, and had widespread transfer in contralateral chest and the lung. The median survival time of the orthotopic group （ 38, 35, 23 days） were less than the heterotopic group （82, 72, 50 days）. MVD of the orthotopic group （120.2±9.73） was higher than the heterotopic group （92.6±7.12）. The expression of CD44v of orthotopic （26.46±1.56）% was higher than the heterotopic group （23.13±1.02）%. Conclusion The lung cancer orthotopic model which established by 3LL cells transplanted into the lung of the mice is simple, dependable, repeatable and has stronger transfer characteristics than the heterotopic model.

关 键 词：肺肿瘤 动物模型 小鼠 

分 类 号：R734.2[医药卫生—肿瘤]