氢化物发生原子荧光光谱法测定螺旋藻中无机硒和有机硒  

Determination of Organic and Inorganic Selenium in Spirulina by Hydride Generation-Atomic Florescence Spectrophotometry

作　　者：贺与平[1] 李维香 何素芳[1] 熊敏[1] 单云[1] 刘春侠[1] 

机构地区：[1]昆明理工大学分析测试研究中心,昆明市学府路253号650093 [2]云南省粮油产品质量监督检验所,昆明市650041

出　　处：《光谱实验室》2009年第6期1404-1408,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：云南省教育厅科学研究基金(项目编号:08Z0016)

摘　　要：螺旋藻试样经硝酸、高氯酸消解,原子荧光光谱法测定总硒含量;用水浸提螺旋藻试样,经环己烷萃取后,水相采用硝酸、高氯酸消解后,原子荧光光谱法测定无机硒含量,间接测定螺旋藻中有机硒含量。总硒测定精密度在1.05%—2.10%之间,加标回收率在96.6%—102.3%之间;无机硒的浸提率在95.1%—100.7%之间,测定精密度在2.37%—3.70%之间,加标回收率在93.6%—98.4%之间,检出限为0.47mg/kg,满足螺旋藻中有机硒含量的测定。The total selenium in spirulina was digested by mixed acid of HNO3 and HClO4 and determinated by hydride generation-atomic florescence spectrophotometry （HG-AFS）; After extracting the spirulina by water and then by cyclohexane,the water phase was digested by HNO3 and HClO4,and the inorganic selenium was determinated by HG-AFS,consequently,the content of organic selenium was calculated. The accuracy of total Se is 1.05%--2.10% ,and the recovery is 96. 6%- 102.3%. The extraction rate of inorganic Se is 95. 1%--100. 7%,the accuracy of inorganic Se is 2.37%--3. 70%,and the recovery of inorganic Se is 93. 6%--98.4%,the detection limit is 0. 47 mg/kg. This method meets the determintion of organic selenium in spirulina.

关 键 词：有机硒 无机硒 螺旋藻 氢化物发生-原子荧光光谱法 

分 类 号：O657.31[理学—分析化学]