小剂量化疗药物对胶质瘤母细胞U87的生长抑制作用  

Inhibitory effect of low-dose chemotherapy drugs on the growth of glioblastoma cell line U87

作　　者：崔向丽[1] 赵志刚[1] 弓建华[2] 许艳妮[2] 贾文清[3] 林松[3] 

机构地区：[1]首都医科大学附属北京天坛医院药剂科,北京100050 [2]中国医学科学院医药生物技术研究所,北京100050 [3]首都医科大学附属北京天坛医院神经外科,北京100050

出　　处：《中国临床药理学杂志》2009年第6期487-491,共5页The Chinese Journal of Clinical Pharmacology

基　　金：科技部973基金资助项目(2007CB512503)

摘　　要：目的研究体外小剂量化疗药物尼莫司汀(ACNU)、顺铂(CDDP)、卡铂(CBP)(均抗癌药)持续给药对人胶质母细胞瘤株U87的生长抑制作用。方法不同浓度的ACNU、CDDP、CBP分别作用于U87,24h后撤药(A组)和持续作用于U8772h(B组),用MTT法在24、48、72h分别检测药物的2种作用方式对U87细胞的生长抑制率。结果不同浓度ACNU的2种方式,作用48、72h对U87的生长抑制率差异无显著性(P>0.05);不同浓度CDDP和CBP持续作用48、72h(B组)对U87的生长抑制率显著高于24h撤药组(A组),2组比较差异性显著(P<005)。结论小剂量化疗药物在一定的浓度范围内持续作用,对U87的体外生长抑制作用与药物浓度和作用时间成正比。Objective To investigate the continous inhibitory effect of low dose nimustine （ACNU）, cisplatin（CDDP） and carboplatin（CBP） on the growth of glioblastoma cell line U87 in vitro. Methods U87 cells are divided into 2 groups： One mode is 24 h incubation of U87 with different concentration of ACNU, CDDP and CBP. DMEM was discarded, and incubate for another 24 h or 48 h in fresh DMEM without drugs （ A group）. Another mode is U87 incubated with ACNU, CDDP and CBP respectively. The growth inhibition rate of U87 was detected respectively by MTT assay in 24 h, 48 h and 72 h （ B group）. Results The inhibitory rate of ACNU on the growth of U87 by 2 incubation modes of 48 h and 72 h had no differenee（P 〉0. 05）. The inhibitory rate of CDDP and CBP on the growth of U87 by eontinous incubation group （B group） is much higher than the group with discard drugs after 24 h （ A group） after 48 h and 72 h incubation. The difference of the 2 groups is prominent （ P 〈 0. 05 ）. Conclusion The inhibitory rate on the growth of U87 in vitro is proportional to the concentration and time of low dose chemotherapy drugs.

关 键 词：胶质母细胞瘤 尼莫司汀 顺铂 卡铂 

分 类 号：R735.2[医药卫生—肿瘤] R739.704[医药卫生—临床医学]