染色体22q12上THOC5和ADRBK2基因在人脑胶质瘤组织中mRNA表达  

The mRNA expression of THOC5 and ADRBK2 gene which located at chromosome 22q12 in human gliomas

作　　者：田宇[1] 王锦鸿[2] 綦斌[2] 胡国章[1] 李朝晖[1] 祝子峰[1] 王欣[3] 

机构地区：[1]吉林大学中日联谊医院神经外科,吉林长春130033 [2]吉林大学第一医院 [3]吉林大学第二医院

出　　处：《中国实验诊断学》2010年第2期183-185,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(30672159);教育部"新世纪优秀人才支持计划"(NCET-06-0306);吉林省科技厅科学基金资助项目(200506161)

摘　　要：目的观察位于染色体22q12上的THOC5和ADRBK2基因在人脑胶质瘤肿瘤组织中mRNA表达。方法应用逆转录-聚合酶链反应(RT-PCR)及图像分析方法,检测17例人脑胶质瘤肿瘤组织和8例正常人脑组织中THOC5和ADRBK2基因mRNA表达。基因表达水平用THOC5或ADRBK2基因/β-肌动蛋白(β-actin)灰度比值×100表示。结果ADRBK2基因在正常人脑组织和人脑胶质瘤肿瘤组织中,均存在弱表达,统计分析显示无显著性差异(P>0.05);THOC5基因在正常人脑组织中弱表达,在人脑胶质瘤肿瘤组织中高表达。统计分析显示:人脑胶质瘤肿瘤组织中THOC5基因mRNA表达与正常人脑组织中THOC5基因mRNA表达比较,有显著性差异(P<0.05)。结论THOC5基因高表达可能与胶质瘤的发生、发展相关。Objective To obserev the mRNA expression of THOC5 and ADRBK2 genes in human gliomas. Methods The mRNA expression of THOC5 and ADRBK2 genes in 17 cases gliomas and 8 cases normal brain tissues were detected by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） in the presence of specific primers. The level of THOC5 and ADRBK2 gene expression were expressed as ratio expression rate （RER） of THOC5 or ADRBK2 gene/β-actin 100. Results ADRBK2 gene had weak expression in normal brain tissues and gliomas, and there was no significanted difference（ P 〉 0.05） ;THOC5 gene had weak expression in normal brain tissues, had significantic expression in gliomas. There was significanted difference of THOC5 gene between normal brain tissues and gliomas（ P 〈 0.05）. Conclusion It can be inferred that the overexpression of THOC5 gene may be involved in glioma orgin.

关 键 词：胶质瘤 THOC5基因 ADRBK2基因 逆转录-聚合酶链反应 

分 类 号：R739.41[医药卫生—肿瘤]