毛细管电泳非接触电导检测法研究电渗流峰变化规律  

Study on Electroosmotic Flow by Capillary Electrophoresis with Contactless Conductivity Detection

作　　者：陈昌国[1] 李雷光[1] 尧玉芬[1] 雷淑[1] 

机构地区：[1]重庆大学化学化工学院,重庆400044

出　　处：《分析科学学报》2010年第1期43-46,共4页Journal of Analytical Science

基　　金：天津市科技支撑计划重点项目(No.07ZCKFGX01800)

摘　　要：采用自行研制的非接触电导检测器,结合毛细管电泳技术,以10 mmol/L的2-N-吗啡啉乙磺酸(MES)/L-组氨酸(L-His)为缓冲溶液,系统研究了缓冲溶液浓度、pH值、样品浓度对电渗流峰的变化规律。结果表明:随着样品KCl溶液的浓度的减小,电渗流峰出现由正峰逐渐变为负峰的现象,并结合非接触电导检测原理、L-His的特性和质子平衡方程进行了的解释。The influence of sample concentration and buffer solution on eleetroosmotic flow （EOF） was studied by capilly eleetrophoresis with contactless conductivity detection, in which 2-（N-Morpholino） ethanesulfonic acid L-histidine was used as the separation buffer solution. The EOF peak transfered from positive to negative with the decrease of the concentration of KCl solution. This phenomenon could be explained with the principle of contactless conductivity detection and proton ionization equilibria of L- His.

关 键 词：电渗流峰 毛细管电泳非接触电导检测 L-组氨酸 

分 类 号：O657.8[理学—分析化学]