胆管癌中MRP-1和GST-π的表达及临床意义  

Expression of multidrug resistance-related genes MRP-1/GST-π in carcinoma of cholangiocarcinoma and its significance

作　　者：安杰[1] 刘伟[2] 耿建英[1] 

机构地区：[1]中国人民解放军白求恩国际和平医院病理科,石家庄市050082 [2]中国人民解放军白求恩国际和平医院普通肝胆外科,石家庄市050082

出　　处：《河北医药》2010年第2期150-152,共3页Hebei Medical Journal

摘　　要：目的多药耐药性(MDR)是导致胆管癌化疗疗效不佳的主要原因,本研究通过检测胆管癌组织中MRP-1和GST-π的表达情况,探讨其耐药的临床意义。方法收集2001年1至2007年12月收治并行手术切除的21例胆管癌标本,RT-PCR方法检测标本中MRP-1及GST-π mRNA的表达。结果MRP-1和GST-π mRNA在胆管癌组织中的表达阳性率分别76.2%和66.7%,显著高于对照组(P<0.05),上述指标与性别、年龄、病理分期、分化程度、淋巴结转移均无关(P>0.05)。结论MRP-1、GST-π基因在未经过化疗的胆管癌组织中均有不同程度的高表达;胆管癌的原发性多药耐药可能与MRP-1、GST-π有关。Objective Multidrug resistance is the main cause for the patients with cholangiocarcinoma for not responding to chemotherapy. To investigate the gene expression of multidrug resistance-related protein 1 （MRP-1）, glutathione S-transferase-IT（GST-IT） in cholangiocarcinoma in order to explore the clinical significance of multidrug resistance. Methods The expression of MRP-1 and GST-IT in 21 cases of cholangiocarcinoma was measured by RT-PCR method, while 8 cases of choledochitis served as the control group. Results The positive rates of MRP-1, GST-π mRNA in cholangiocarcinoma were 76.2% ,66.7 % respectively, which were significantly higher than those of control group （ P 〈 0. 05 ）. Their expressions were not related to gender, age, staging, differentiation and lymph node metastasis （ P 〉 0.05 ）. Conclusion MRP-1 and GST-π are overexpressed in various degrees of cholangiocarcinoma without chemotherapy, which may be related to the primary muhidrug resistance of the tumors.

关 键 词：胆管癌 多药耐药相关蛋白 谷胱苷肽S转移酶-π 

分 类 号：R735.8[医药卫生—肿瘤]