ATRA诱导胶质瘤C6细胞凋亡过程中Bcl-2和Caspase-3表达的研究  

Expression of Bcl-2 and Caspase-3 in apoptosis induced by all-trans-retinoic acid of C6 glioma cells in rat

作　　者：李娟[1] 张磊[1] 费舟[1] 章翔[1] 甄海宁[1] 张晓楠[2] 霍军丽[1] 

机构地区：[1]第四军医大学西京医院全军神经外科研究所,陕西西安710032 [2]第四军医大学基础部药理教研室,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2010年第1期23-26,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(30672371)

摘　　要：目的研究全反式维甲酸(ATRA)诱导胶质瘤C6细胞凋亡过程中Caspase-3和Bcl-2表达情况,探讨ATRA诱导胶质瘤C6细胞凋亡的机制。方法用四甲基偶氮唑蓝(MTT)比色法绘制ATRA不同浓度不同时间(6、12、24、48、72h)对胶质瘤C6细胞作用后细胞生长曲线,逆转录酶-多聚酶链反应(RT-PCR)检测凋亡相关基因Bcl-2和Caspase-3 mRNA水平的表达变化影响,Western blot分析Caspase-3和Bcl-2在胶质瘤C6细胞中的表达情况。结果胶质瘤C6细胞经ATRA诱导后,细胞的增殖明显受到抑制,通过RT-PCR结果证实Bcl-2 mRNA在ATRA作用下明显呈低表达而Caspase-3 mRNA的表达随时间延长逐渐增高,Westernblot检测结果显示ATRA作用后,下调凋亡抑制蛋白Bcl-2的表达并激活了凋亡蛋白Caspase-3的表达。结论ATRA对胶质瘤C6细胞的增殖具有抑制作用。Objective To study the expression of Bcl-2 and Caspase-3 in the apoptosis of rat C6 glioma cells and the mechanism of apoptosis which induced by all-trans retinoic acid （ATRA）. Methods Methyl thiazolyl tetrazolium （MTF） assay was performed to measure the effect on the proliferation of C6 glioma cells treated with different concentrations of ATRA for 6 h, 12 h, 24 h, 48 h and 72 h. And the expression levels of mRNA and protein of Caspase-3 and Bcl-2 in C6 glioma cells were examined by reverse transcriptase-polymerase chain reaction （RT-PCR） and Western-blot. Results ATRA significantly inhibited the cell viability of C6 glioma cells, and the results of RT-PCR and Western-blot showed that ATRA down-regulated the expression of anti-apoptotic protein Bcl-2 and activated the pro-apoptotic protein Caspase-3. Conclusion ATRA can effectively inhibit the proliferation of C6 glioma cells and induce the apoptosis.

关 键 词：全反式维甲酸 胶质瘤C6 BCL-2 CASPASE-3 

分 类 号：R739.41[医药卫生—肿瘤]