高脂饲料结合CCl_4诱导小鼠非酒精性脂肪肝模型的建立  

Establishment of non-alcoholic fatty liver mice model with high-fat feeding and carbon tetrachloride

作　　者：刘锋[1] 向志钢[2] 许继凡[1] 罗婧[1] 石小凤[1] 王苏燕[1] 

机构地区：[1]吉首大学医学院临床医学系,湖南416000 [2]吉首大学医学研究所

出　　处：《江苏医药》2010年第1期69-71,F0003,共4页Jiangsu Medical Journal

基　　金：湖南省自然科学基金(07JJ6029);吉首大学校级科研项目(08JDX027);吉首大学大学生研究性学习和创新性实验计划项目(教通[2008]22)

摘　　要：目的利用高脂饲料和四氯化碳(CCl4)复合诱导小鼠非酒精性脂肪肝(NAFLD)模型。方法40只昆明小鼠随机均分为4组:普通饲料+生理盐水(A)组、高脂饲料+生理盐水(B)组、普通饲料+CCl4稀释液(C)组、高脂饲料+CCl4稀释液(D)组,3周后,比较各组血清生化指标及肝组织病理学指标的差异。结果D组小鼠肝指数[(5.5±0.18)%]、TG(2.01±0.27)mmol/L、TC(5.35±0.73)mmol/L、ALT(389.28±18.02)U/L、AST(416.86±30.68)U/L,均明显高于A、B、C组(P<0.05);C组小鼠血清ALT、AST明显高于A组(P<0.05)。镜下D组大部分肝细胞脂肪变性,脂滴大量成群出现且严重聚积,与其他各组差异显著。结论通过3周的高脂饲料与CCl4复合诱导,可快速建立小鼠NAFLD模型。Objective To establish a non-alcoholic fatty liver model（NAFLM）in mice by high-fat feeding and carbon tetrachloride.Methods Fourty Kunming mice were randomly divided into 4 groups with 10 mice each.The mice were fed with common food plus normal saline（group A）,high-fat plus normal saline（group B）,common food plus carbon tetrachloride（group C）,or high-fat food plus carbon tetrachloride（group D）.After three weeks,all mice were killed.The liver index,serum biochemical criteria and hepatic histological changes were compared.Results The liver index [（5.5±0.18）%）],serum TG [（2.01±0.27）mmol/L],TC [（5.35±0.73（mmol/L],ALT [（389.28±18.02（U/L],AST [（416.86±30.68（U/L] of group D were all higher than those of groups of A,B,C（P〈0.05）.Compared with group A,the serum AST and ALT of group C were higher those of group A（P〈0.05）.Compared with other groups,a lot of hepatocellular diffuse steatosis and a large quantity of the fat drop appeared and accumulated seriously in group D.Conclusion The NAFLM of mice can be established successfully by feeding with high-fat food and injecting carbon tetrachloride for 3 weeks.

关 键 词：非酒精性脂肪肝 四氯化碳 动物模型 

分 类 号：R575[医药卫生—消化系统]