miRNA表达谱改变与卵巢癌转移潜力相关性的研究  

Different microRNA profiles related with ovarian cancer metastasis

作　　者：王悦[1] 董丽[2] 崔恒[1] 白符[1] 付天云[1] 姚远洋[1] 

机构地区：[1]北京大学人民医院妇科,北京100044 [2]北京大学积水潭医院妇科

出　　处：《现代妇产科进展》2010年第1期1-6,共6页Progress in Obstetrics and Gynecology

基　　金：北京大学人民医院2007年度研究与发展基金资助(No:RDB2007-9)

摘　　要：目的:探讨卵巢癌转移的相关机制,检测有不同侵袭力卵巢癌细胞的miR-NA差异表达谱。方法:应用包含924条成熟miRNA探针的哺乳动物miRNA芯片V3.0杂交检测具有不同侵袭力的人卵巢乳头状腺癌SKOV3.ip1细胞与其母系SKOV3细胞miRNA表达谱的差异,并与cDNA芯片基因检测结果进行比对分析。结果:miRNA芯片检测到39个2倍以上差异表达miRNA,对比SKOV3细胞,SKOV3.ip1细胞中有11个miRNA表达上调和28个miRNA表达下调,其中包括了hsa-miR-200a/b、-10b、-34a、-224、-148a等已知与侵袭转移相关的重要miRNA。比对cDNA芯片检测结果,提供了IG-FBP1、VEGFB、NME1等重要侵袭相关基因可能调控miRNA的机制。结论:卵巢癌侵袭转移过程中,多个miRNA参与其中,担当重要的调控作用。miRNA有望成为卵巢癌转移的标记物和治疗的靶点。Objective：To explore the mechanism of metastasis of ovarian cancer, the difference of mieroRNA（miRNA） profiles between two ovarian cancer cells having different invasion potentials. Methods：The miRNA expression profiles of human ovarian epithelial papillary adenocarcinoma cells with different invasion potential, SKOV3. ip1 and its maternal cell SKOV3, were compared using a mammalian miRNA mieroarray version 3.0 which containing 924 mature miRNA sequences. The results were analyzed and compared with previous human genomic eDNA array results of these two cells. Results：39 miRNAs with different expression level over 2-fold were identified. Compared with that in SKOV3,11 miRNAs were found up-regulated and 28 miRNAs were found down-regulated in SKOV3. ip1. hsa-miR-200a/b,-10b,-34a,-224 and -148a were known as important genes associated with metastasis. The analysis together with the findings in previous cDNA array provided the clue of the potential regulation mechanism of miRNAs to cancer cell invasion related genes, such as IGFBP1, VEGFB, NME1 and other genes. Conclusions：Various miRNAs may involve in regulation of the ovarian cancer invasion and metastasis related genes or signal pathways. Moreover, miRNAs may take an important role in it. miRNAs would be the valuable markers for metastasis monitoring and therapeutic targets for ovarian cancer.

关 键 词：卵巢肿瘤 MIRNA MIRNA芯片 肿瘤转移 

分 类 号：R737.31[医药卫生—肿瘤]