7.0T MRI及Micro-PET无创检测小鼠动脉粥样硬化  

Noninvasive observation of atherosclerosis in mice with 7.0T MR and Micro-PET

作　　者：王中娟[1] 邓钢[1] 黄洪波[2] 李爱梅[3] 居胜红[1] 赵瑞[1] 金晖[1] 魏晓莹[1] 

机构地区：[1]东南大学附属中大医院放射科,江苏南京210009 [2]江苏省原子能医学研究所卫生部核医学重点实验室,江苏无锡214063 [3]南京大学医学院附属鼓楼医院PET/CT中心,江苏南京210009

出　　处：《中国医学影像技术》2010年第2期209-212,共4页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金(30770624;30830039)

摘　　要：目的探讨7.0T小动物MRI及Micro-PET检测动脉粥样硬化的可行性。方法取10只46周龄ApoE-/-小鼠建立老年ApoE-/-小鼠动脉粥样硬化模型,取5只模型于鼠尾静脉注射超顺磁性氧化铁(SPIO)前、后12h、24h、36h行MR扫描(应用7.0TMR仪);另5只模型于鼠尾静脉注射18F-氟脱氧葡萄糖(18F-FDG)1h、2h、3h后行Micro-PET显像;取腹主动脉行病理检查。结果老年ApoE-/-小鼠高脂喂养6个月后,7.0TMRI证实动脉粥样硬化斑块形成;鼠尾静脉注射SPIO后36h发现鼠腹主动脉及两侧髂动脉血管壁T2W像上环形高信号较未注射SPIO前变薄,血管腔较前增宽。鼠尾静脉注射18F-FDG行Micro-PET检测,发现在3h左右腹主动脉及两侧髂动脉区域有放射性浓聚。病理示腹主动脉壁内有动脉粥样硬化斑块形成及巨噬细胞的聚集。结论7.0TMRI及Micro-PET可成功检测巨噬细胞丰富的动脉粥样硬化斑块,有助于判断斑块的易损性,为早期发现、诊断及治疗动脉粥样硬化提供理论依据。Objective To explore the feasibility of detecting atherosclerosis with 7.0T MR and Micro-PET. Methods Ten 46-week-old ApoE-/- mice with high lipid diet for 6 months were selected to establish atherosclerosis models. Among them, 5 mice underwent MRI before and 12 h, 24 h, 36 h after injection of SPIO, respectively, and the other 5 mice were injected with ^18F-fluorodeoxyglucose （^18F-FDG） through tail vein and observed with Micro-PET after 1 h, 2 h and 3 h. The specimens of abdominal aorta were taken for pathologic examination. Results Atherosclerotic plaques were observed in all animals with 7.0T MRI after 6 months high lipid diet. Thirty-six hours after the injection of SPIO, the high signal rings were thinner and the lumen of blood vessels were wider than those before injection on T2WI. Radioactive concentration was observed in abdominal aorta and both sides of iliac artery 3 h after the injection of ^18 F-fluorodeoxyglucose （18 F-FDG）. Pathological examination showed the formation of atherosclerotic plaques and the aggregation of the macrophages. Conclusion 7.0T MRI and Micro-PET can be used to observe the macrophage-rich plaque and to judge the vulnerability of plaque, thus provide theoretical basis for early detection, diagnosis and treatment of atherosclerosis.

关 键 词：动脉硬化 巨噬细胞 磁共振 Micro-PET APOE-/-小鼠 

分 类 号：R-332[医药卫生] R445.2