Syk基因在食管鳞癌组织中的表达及临床意义  

Clinical significance of Syk expression in human esophageal squamous cell carcinoma

作　　者：刘泓基[1] 臧真真[1] 侯巧燕[2] 陆竞艳[2] 杨峻[3] 林中[1] 赵永忠[1] 

机构地区：[1]桂林医学院附属医院消化科,广西壮族自治区桂林市541001 [2]桂林医学院附属医院病理科,广西壮族自治区桂林市541001 [3]桂林医学院附属医院检验科,广西壮族自治区桂林市541001

出　　处：《世界华人消化杂志》2010年第1期33-38,共6页World Chinese Journal of Digestology

基　　金：广西壮族自治区卫生厅自筹基金资助项目;No.Z2007220~~

摘　　要：目的:分析食管鳞癌组织及其对应癌旁组织中Syk蛋白和mRNA的表达,探讨其与食管鳞癌临床恶性生物学行为的关系.方法:采用免疫组织化学法检测48例食管鳞癌组织及其对应癌旁组织中Syk蛋白的表达;RT-PCR法检测43例食管鳞癌组织及其对应癌旁组织中SykmRNA的表达,并观察其与食管鳞癌患者肿瘤大小、TNM分期、淋巴结转移的关系.结果:食管鳞癌组织Syk蛋白表达阳性率显著低于癌旁组织(16.67%vs89.58%,P<0.05);Syk蛋白表达与肿瘤TNM分期相关(χ2=6.713,P<0.05);Syk在淋巴结转移组中的阳性表达率显著低于无淋巴结转移组(3.03%vs29.41%,P<0.05);Syk表达与肿瘤大小无关(χ2=0.017,P>0.05).食管鳞癌组织中SykmRNA的表达量明显低于其在对应癌旁组织中的表达量(t=-11.27,P<0.05).结论:Syk蛋白和mRNA在食管鳞癌中表达缺失与其发生及转移倾向相关,Syk可能是食管鳞癌的肿瘤抑制基因,可能作为分子标记而用于食管鳞癌的早期诊治.AIM： To investigate the expression of spleen tyrosine kinase （Syk） mRNA and protein in human esophageal squamous cell carcinoma （ESCC） and analyze its correlation with the malignant biological behavior of ESCC. METHODS： The expression of Syk protein in 48 ESCC specimens and matched adjacent noncarcinoma specimens was detected by immunohistochemistry. The expression of Syk mRNA in 43 ESCC specimens and matched adjacent noncarcinoma specimens was assayed by reverse transcription-polymerase chain reaction （RTPCR）. The correlations of Syk expression with tumor size, TNM stage and lymph node metastasis were analyzed. RESULTS： The positive rate of Syk protein expression in ESCC tissue was significantly lower than that in adjacent non-carcinoma tissue （16.67% vs 89.58%, P 〈0.05）. The expression of Syk protein was correlated with tumor TNM stage in ESCC （χ^ 2 = 6.713, P 〈0.05）. A significant difference was noted in Syk expression between ESCC with and without lymph node metastasis （3.03% vs 29.41%, P 〈0.05）. No significant correlation was observed between Syk expression and tumor size （χ^ 2 = 0.017, P 〉0.05）. RT-PCR analysis showed that the expression level of Syk mRNA was significantly lower in ESCC than in adjacent non-carcinoma tissue （t =-11.27, P 〈0.05）. CONCLUSION： Downregulation of Syk gene expression may be involved in the carcinogenesis and metastasis of ESCC. Syk gene is a novel candidate tumor suppressor gene in ESCC and can be used as a molecular marker for early diagnosis and treatment of ESCC.

关 键 词：食管鳞癌 脾酪氨酸激酶 免疫组织化学 逆转录-聚合酶链反应 抑癌基因 

分 类 号：R735.1[医药卫生—肿瘤] R737.9[医药卫生—临床医学]