大鼠肠道DCs及相关细胞因子在冷-束缚应激诱导肠功能紊乱后的表达  

Expression of CD11c, VIP and IL-1β in the intestine and peripheral blood of rats with intestinal dysfunction induced by cold-restraint stress

作　　者：姜道亮[1] 王巧民[1] 宋继中[1] 周政[1] 徐雪梅[1] 代子艳[1] 

机构地区：[1]安徽医科大学附属省立医院消化内科,安徽省合肥市230001

出　　处：《世界华人消化杂志》2010年第1期89-93,共5页World Chinese Journal of Digestology

基　　金：安徽省教育厅科研基金资助项目;NoKJ2008B11ZC~~

摘　　要：目的:了解肠道表面树突状细胞(DCs)及炎症细胞因子VIP、IL-1β在肠功能紊乱时的表达,以及外周血中VIP、IL-1β的表达,探讨大鼠肠道免疫耐受的变化.方法:将60只Wistar大鼠随机分为实验组和对照组,建立冷-束缚应激动物模型.将大鼠处死后,取大鼠回肠末端、远端结肠各长约2cm的肠段,免疫组织化学法检测肠道表面CD11c、VIP、IL-1β的表达,留取腹主动脉血清用ELISA方法检测外周血清中VIP、IL-1β的表达.结果:在回肠末端,实验组大鼠CD11c的表达较对照组无显著差异性;实验组大鼠VIP、IL-1β表达较对照组均明显增加,差异有显著性(177.67±35.44vs92.64±22.19,359.56±45.48vs216.46±41.56,均P<0.05).并且VIP的表达与IL-1β呈正相关(r=0.78,P<0.01).在远端结肠,实验组大鼠CD11c、IL-1β的表达较对照组均无显著差异性;而实验组大鼠远端结肠VIP的表达较对照组明显增高,差异有显著性(380.15±33.24vs254.04±40.53,P<0.05);在外周血中,与对照组相比较,VIP、IL-1β表达均增高,差异有显著性(149.03ng/L±56.82ng/Lvs104.24ng/L±39.03ng/L,8.82ng/L±3.91ng/Lvs5.49ng/L±3.79ng/L,P<0.05).结论:冷-束缚应激后肠功能紊乱大鼠中,末端回肠和远端结肠黏膜存在轻度的炎症反应,DCs尚不能解释肠功能紊乱后肠道存在轻度炎症反应的发病机制.AIM： To investigate the expression of CD11c, vasoactive intestinal peptide （VIP） and interleukin-1β （IL-1β） in the intestine and peripheral blood of rats with intestinal dysfunction induced by cold-restraint stress, and analyze the changes in intestinal immune tolerance in these rats. METHODS： Sixty Wistar rats were randomly divided into control group and experimental group. Intestinal dysfunction was induced in rats by cold-restraint stress. The macroscopic and histological changes in the terminal ileum and distal colon were scored. Immunohisto-chemistry was used to detect the expression of CD11c, VIP and IL-1β in the intestine. The levels of VIP and IL-1β in peripheral blood was measured by enzyme-linked immunosorbent assay （ELISA）. RESULTS： Although there was no significant difference in the expression of CD11c in the terminal ileum between the experimental group and the control group, the expression levels of VIP and IL-1β in the terminal ileum in the experimental group were significantly higher than those in the control group （177.67 ± 35.44 vs 92.64 ± 22.19, and 359.56 ± 45.48 vs 216.46 ± 41.56, respectively; both P〈0.05）. A positive linear correlation was noted between the expression of VIP and IL-1β in the terminal ileum （r = 0.78, P〈0.01）. No significant differences were noted in the expression levels of CD11c and IL1β in the distal colon between the experimental group and control group, whereas the expression level of VIP in the experimental group was significantly higher than that in the control group （380.15 ± 33.24 vs 254.04 ± 40.53, P〈0.05）. Compared with the control group, the levels of VIP and IL-1β in peripheral blood increased significantly in the experimental group （149.03 ± 56.82 ng/L vs 104.24 ± 39.03 ng/L, and 8.82 ± 3.91 ng/L vs 5.49 ± 3.79 ng/L, respectively; both P〈0.05）. CONCLUSION： Low-grade inflammation is noted in the terminal ileum and distal colon of rats with intestinal dysfunction induced by cold-restraint stress. Dentr

关 键 词：应激 树突状细胞 肠功能紊乱 

分 类 号：R574[医药卫生—消化系统]