EIF-5A2在肝细胞癌中的表达及其与VEGF、MVD的关系  

Correlations of EIF-5A2 protein expression with VEGF expression and microvessel density in hepatocellular carcinoma

作　　者：赖旭东[1] 陈晓辉[2] 许贺标[3] 刘玉荣[3] 马宁芳[3] 

机构地区：[1]广州市红十字会医院急诊科,广东省广州市510220 [2]广州医学院第一附属医院急诊科,广东省广州市510182 [3]广州医学院组织学与胚胎学教研室,广东省广州市510260

出　　处：《世界华人消化杂志》2010年第2期137-142,共6页World Chinese Journal of Digestology

基　　金：广州市教育局高校科技计划基金资助项目;No.61053~~

摘　　要：目的:探讨真核翻译起始因子-5A2(eukaryotic initiation factor 5A2,EIF-5A2)在肝癌内的表达及其与VEGF表达、血管密度和临床病理指标的关系.方法:用免疫组织化学方法检测49例HCC组织及6例正常肝组织中EIF-5A2、VEGF和CD34的表达,计数微血管密度(MVD),并结合临床病理特征进行比较分析.结果:49例HCC组织中EIF-5A2、VEGF、CD34阳性表达率分别为87.7%、89.7%及100.0%,正常肝组织EIF-5A2、CD34表达呈阴性,VEGF表达为阴性或弱阳性.肝癌组织中EIF-5A2蛋白表达与VEGF表达及MVD间均呈正相关(r=0.416,0.321,均P<0.05);不同肿瘤灶数量的HCC组织EIF-5A2、VEGF蛋白表达及MVD差别无显著性,不同直径HCC的EIF-5A2、VEGF蛋白表达差别亦无显著性,而不同直径的HCC的MVD差别有显著性(P<0.05).有癌栓形成组与无癌栓组相比EIF-5A2、VEGF蛋白表达及MVD差别均有显著性(均P<0.05).肿瘤包膜完整组与无胞膜/包膜不完整组两组间EIF-5A2、VEGF蛋白表达及MVD差别均有显著性(均P<0.05).结论:肝癌组织中有较高的EIF-5A2阳性表达;EIF-5A2表达与VEGF表达及MVD呈正相关,与HCC组织血管侵犯和转移倾向有关.AIM：To investigate the correlations of eukaryotic initiation factor-5A2 （EIF-5A2） expression with vascular endothelial growth factor （VEGF） expression,microvessel density （MVD） and clinicopathological parameters in hepatocellular carcinoma （HCC）.METHODS：Immunohistochemistry was used to determine the expression of EIF-5A2,VEGF and CD34 in 49 HCC tissues and 6 normal liver tissues.The MVD was calculated by counting CD34-labeled vessels.The correlations of EIF5A2 expression with VEGF expression,MVD and clinicopathological parameters in HCC were then analyzed.RESULTS：The positive rates of EIF-5A2,VEGF and CD34 expression in 49 HCC tissues were 87.7%,89.7% and 100%,respectively.In contrast,EIF-5A2 and CD34 expression was not detected and VEGF was weakly detected in normal liver tissues.EIF-5A2 expression was positively correlated with VEGF expression and MVD （r=0.416 and 0.321,respectively;both P 〈0.05） in HCC.There were no significant differences in EIF-5A2 expression,VEGF expression and MVD between HCC tissues containing a single tumor focus and multiple tumor foci.No differences were found in the expression of EIF-5A2 and VEGF among HCCs of different diameters though a significant difference was noted in MVD.The levels of EIF-5A2 and VEGF expression and MVD were significantly higher in HCC patients with portal vein tumor thrombosis than in those without portal vein tumor thrombosis （all P〈 0.05）.Significant differences were also noted in EIF-5A2 and VEGF expression and MVD between HCC with an integral capsule and that without an integral capsule （all P〈 0.05）.CONCLUSION：EIF-5A2 is highly expressed in HCC tissues.EIF-5A2 expression is positively correlated with VEGF expression and MVD in HCC.The overexpression of EIF-5A2 may play an important role in portal vein encroachment and metastasis of HCC.

关 键 词：真核翻译起始因子-5A2 血管内皮生长因子 微血管密度 肝细胞癌 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]