电离辐射诱发小鼠胸腺淋巴瘤基因表达谱改变  

Differential gene expression profile induced by ionizing radiation in thymic lymphoma of mice

作　　者：刘永哲[1] 于雷[1] 孙世龙[1] 杨湘山[1] 武宁[1] 鞠桂芝[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,长春130021

出　　处：《中国公共卫生》2010年第2期222-223,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30670630)

摘　　要：目的探讨电离辐射诱发小鼠胸腺淋巴瘤基因表达谱的改变。方法1.75 Gy X射线每周1次,分4次照射BALB/c小鼠建立电离辐射诱发小鼠胸腺淋巴瘤模型,小鼠Mouse WG-6 v2.0全基因组表达谱微珠芯片筛选与正常小鼠胸腺差异表达基因,选取较重要的基因实时定量PCR验证。结果共筛选出3 063个差异表达基因,其中上调基因1 591个,下调基因1 472个,涉及KEGG数据库145个通路,BioCarta数据库76个通路及GenMAPP数据库218个通路;实时定量PCR结果显示,电离辐射诱发的小鼠胸腺淋巴瘤Bcl11b、Ikzf1基因mRNA表达水平均有下调趋势。结论电离辐射诱发小鼠胸腺淋巴瘤基因表达谱发生改变,涉及大量通路。Objective To investigate the differences of gene expression induced by ionizing radiation in thymic lymphoma of mice. Methods BALB/c mice were subjected to whole body irradiation with 1. 75Gy X-ray once a week for 4 weeks to induce thymic lymphoma. The difference of gene expression in thymic ceU of mice was detected by Mouse WG-6 v2.0 Expression BeadChip. The real-time PCR was used to identify important genes. Results The chip depicted 3 063 genes with differential gene expressions including 1 591 up-regulation and 1 472 down-regulation genes. The genes related to 145 pathways of KEGG database,76 pathways of BioCarta database and 218 pathways of GenMAPP database. The results of real-time PCR showed that the mRNA expression level of Bcll 1 b and lkzfl genes tended to be down-regulated. Conclusion The ionizing radiation may induce differential gene expression profile in thymic lymphoma of mice, and the process may involve many pathways.

关 键 词：电离辐射 胸腺淋巴瘤 基因表达谱 

分 类 号：Q345.2[生物学—遗传学] R733[医药卫生—肿瘤]