重组人白细胞介素3和白细胞介素6对骨髓基质细胞生长的调节作用  

Regulative effects of rhIL 3 and rhIL 6 on proliferation of stromal cells from marrow

作　　者：郝思国[1,2,3,4] 杨吉成[1,2,3,4] 邹正辉 盛伟华[1,2,3,4] 张硕 

机构地区：[1]安徽医科大学第一附属医院血液科 [2]苏州医学院基因工程研究室 [3]苏州医学院基因附属二院血液科 [4]安徽省合肥国家建材局水泥研究院卫生所

出　　处：《安徽医科大学学报》1998年第6期425-427,共3页Acta Universitatis Medicinalis Anhui

摘　　要：目的了解重组人白细胞介素３（ｒｈＩＬ－３）和重组人白细胞介素６（ｒｈＩＬ－６）对骨髓造血微环境的调节作用。方法应用成纤维细胞体外集落形成法，观察了ｒｈＩＬ－３和ｒｈＩＬ－６对成纤维细胞集落（ＣＦＵ－Ｆ）体外生长的影响。结果ｒｈＩＬ－３和ｒｈＩＬ－６能显著地刺激ＣＦＵ－Ｆ的生长，两者联合应用时对ＣＦＵ－Ｆ生长的刺激作用明显强于两者单用，表现出协同效应。结论ｒｈＩＬ－３和ｒｈＩＬ－６可促进基质细胞生长。Objective To determine the regulative effects of recombinant human interleukin 3(rhIL 3) and interleukin 6(rhIL 6) on bone marrow hematopoietic microenvironment. Methods The effects of rhIL 3 and rhIL 6 on proliferation of colony forming Unit Fibroblasts (CFU F) were studied in vitro CFU F culture system. Results Both of these interleukins can promote the proliferation of CFU F in vitro with a dosage dependence, meanwhile, the synergistic effects between rhIL 3 and rhIL 6 on proliferation of cells from CFU F were observed. Conclusion The findings suggested that rhIL 3 and rhIL 6 may promote the proliferation of marrow stromal cells,which may be helpful for recovery and reconstitution of hematopoietic microenvironment after intensive chemotherapy and bone marrow transplantation.

关 键 词：白血病 成纤维细胞 骨髓移植 RHIL-3 RHIL-6 

分 类 号：R733.7[医药卫生—肿瘤] R457.7[医药卫生—临床医学]