脂质体包裹可溶性蛋白质抗原诱导特异性CTL的研究  

Induction of cytotoxic T lymphocytes with soluble protein antigen entrapped in cationic liposomes

作　　者：管红兵[1] 温文倩[1] 顾青[1] 田毓华[1] 

机构地区：[1]上海市第一人民医院

出　　处：《上海医学》1998年第12期691-693,共3页Shanghai Medical Journal

摘　　要：目的：探讨用可溶性蛋白质抗原诱导特异性ＣＴＬ的途径。方法：用冻融超声法制备包裹可溶性鸡卵蛋白（ＯＶＡ）和ＣＡＨｂ３蛋白质抗原的阳离子脂质体，并借助脂质体将抗原导入Ｃ５７ＢＬ／６小鼠脾细胞胞浆内，以此细胞经尾静脉免疫Ｃ５７ＢＬ／６小鼠，取体内初致敏脾细胞，在体外以渗透方式导入ＯＶＡ或ＣＡＨｂ３抗原的脾细胞再刺激，诱导特异性ＣＴＬ，并进行Ⅰ类途径阻断实验和ＭＨＣＩ类分子中和实验。结果：脂质体成功地包裹了抗原，电镜观察到抗原进入细胞胞浆内，免疫组化检测证实细胞上有抗原表达，细胞毒活性检测显示ＣＴＬ具有抗原特异性和ＭＨＣ限制性，Ⅰ类途径阻断实验证实抗原由Ⅰ类途径提呈，ＭＨＣⅠ类分子中和实验表明ＣＴＬ的识别和杀伤与ＭＨＣⅠ类分子有关。结论：阳离子脂质体能有效地传递可溶性蛋白质抗原，使之进入细胞内抗原Ⅰ类提呈途径，激活特异性ＣＴＬ免疫应答。Objective:To determine whether soluble protein antigen could induce specific CTL response. Methods:Soluble protein antigen OVA and CA Hb3 were encapsulated in cationic liposomes by thaw freeze ultrasonic method and introduced into mice splenocytoplasms, Then injected into C57BL/6 mice through their tail veins,then primed the splenocytes in vivo restimulated in vitro by splenocytes loaded antigen with osmotic means for induction of specific CTL. Blockage of class I pathway and neutrolization of MHC I molecule were also applied. Results:The antigens encapsulated in cationic liposomes and introduced into the cytoplasms were observed under electromicroscope, immunohistochemical assay proved the presence of expression of the antigen on the cells, and cytotoxic ity assay showed that CTL were antigen specific and MHC restricted, Blockage of class I pathway proved that the antigens were presented via class I pathway, neutralization of MHC I molecule indicated the recognition and killing of the CTL were related to MHC I molecule. Conclusions:Soluble protein antigen can be encapsulated in cationic liposomes and introduced into the cytoplasms, which an present through class I pathway, and induce specific CTL responses.

关 键 词：脂质体 鸡卵蛋白 大肠肿瘤 CTL T细胞 

分 类 号：R735.340.2[医药卫生—肿瘤]