佛山地区无偿献血者Rh血型分布情况调查  

Investigation of the distribution of Rh blood group in voluntary blood donors in Foshan,Guangdong province,China

作　　者：伍伟健[1] 罗海玲[1] 黄昌海[1] 郭如华[1] 余晋林[1] 

机构地区：[1]佛山市中心血站,佛山528000

出　　处：《中国免疫学杂志》2010年第2期182-185,共4页Chinese Journal of Immunology

摘　　要：目的:调查并分析佛山地区无偿献血者Rh血型分布情况。方法:使用抗-D、抗-C、抗-c、抗-E和抗-e血清进行Rh血型分型,对RhD抗原筛查阴性的标本使用3批不同厂家IgG抗-D血清采用抗人球蛋白法进行确认。根据实验结果,计算Rh表现型、基因、单倍型频率,并进行Hardy-Weinberg吻合度检验。结果:佛山地区无偿献血者Rh血型表现型频率特点为CCDee>CcDEe>CcDee>ccDEE>ccDEe>CCDEe>CcDEE>ccdee>ccDee,Ccdee>CCdee,ccdEe>CCDEE,CCdEE,CCdEe,CcdEE,CcdEe,ccdEE;单倍型频率特点为CDe>cDE>cDe>CDE;cde>Cde>cdE>CdE;基因频率特点为D>d,C>c,e>E;D(-)表型频率为0.379%,d基因频率为0.061。本文Rh血型单倍型及基因的观察值与期望值差异无统计学意义(P>0.05),Hardy-Weinberg吻合度检验良好。结论:本调查数据符合群体遗传学中的Hardy-Weinberg定律,结果可靠;佛山地区无偿献血人群的Rh血型分布,既有地域特点,也有汉族人群的共性;本研究结果对指导佛山地区科学地进行无偿献血招募及合理地储存血液,提高血站为稀有血型受血者及时供血的能力,具有重要的意义。Objective：To investigate and analysis the distribution situations of Rh blood group system from voluntary blood donors in Foshan area.nethods：Anti-D,anti-C,anti-c,anti-E and anti-e reagents were used to identify Rh blood group system and the RhD（-） was confirmed. The phenotypes, haplotypes and corresponding genotypes frequencies were calculated and the datas were analyzed based on Hardy-Wein- berg law. Results： The characteristics of phenotype frequencies in the voluntary blood donors in Foshan area was CCDee 〉 CcDEe 〉 CcDee 〉 ccDEE 〉 ccDEe 〉 CCDEe 〉 CcDEE 〉 ccdee 〉 ccDee, Ccdee 〉 CCdee, ccdEe 〉 CCDEE, CCdEE, CCdEe, CcdEE, CcdEe and ccdEE. The characteristics of haplotypes frequencies was CDe 〉 cDE 〉 cDe 〉 CDE, de 〉 Cde 〉 cdE 〉 CdE. The characteristics of genes frequencies was D 〉 d, C 〉 c, e 〉 E. The frequency of RhD（-）phenotype was 0. 379 %. The gene frequency of d was 0. 061. The observed value and desired value of the haplotypes and corresponding genotypes were not statistically significant（ P 〉 0.05）. The data were analyzed well based on Hardy-Weinberg law. Conclusion： The result is reliable according to Hardy-Weinberg law. The distribution of Rh blood group system from voluntary blood donors in Foshan area has geographic and general characteristics. The result is important in guiding the recruitment of voluntary blood donors and banking blood reasonably and enhancing the abilitiy of offering blood for the recipients of rare blood group.

关 键 词：佛山地区 无偿献血者 RH血型分布 

分 类 号：R394.5[医药卫生—医学遗传学] R457.11[医药卫生—基础医学]