热休克预处理对TNF-α诱导的RAW264.7巨噬细胞迁移的影响  

Effect of Heat Shock Preconditioning on Raw264.7 Macrophages Migration Induced by TNF-α

作　　者：张丽丽[1,2] 朱珊[1] 刘瑛[1] 刘梅冬[1] 涂自智[1] 刘可[1] 邓恭华[1] 

机构地区：[1]中南大学湘雅医学院病理生理学系,中国湖南长沙410078 [2]长治医学院病理生理学教研室,中国山西长治046000

出　　处：《生命科学研究》2010年第1期50-53,共4页Life Science Research

基　　金：国家"973"计划项目(2007CB512000);国家自然科学基金资助项目(30772022);湖南省自然科学基金资助项目(09JJ4012)

摘　　要：热休克反应是机体中一个重要的内源性保护机制,但其对TNF-α诱导的单核/巨噬细胞迁移有无影响目前尚不清楚.采用酶联免疫吸附实验观察TNF-α(20μg/L)刺激RAW264.7巨噬细胞4h后炎症因子IL-1β、IL-6、IL-15的表达情况;Western blot验证热休克预处理诱导热休克蛋白表达的增加;利用细胞趋化实验观察热休克预处理(42℃,1h)对TNF-α所致巨噬细胞迁移的影响.研究发现,TNF-α可明显促进RAW264.7细胞株中IL-1β、IL-6、IL-15等炎症因子的释放;热休克预处理诱导热休克蛋白HSP70、HSP90、HSP25表达增加;细胞趋化实验发现TNF-α处理的RAW264.7细胞迁移能力较正常对照组明显增强,而热休克预处理组巨噬细胞的迁移能力较单纯TNF-α处理组明显减弱.上述结果表明,热休克预处理抑制TNF-α所致巨噬细胞的迁移.In order to elucidate the effect of heat shock response on TNF-α induced RAW264.7 macropha- ges migration, enzyme linked immuno-sorbent assay（ELISA） was used to observe if TNF-α（20 μg/L） could induce the expression of inflammatory factors IL-1β、IL-6、IL-15; Western Blot assay was used to confirm that Heat shock pretreatment（42 ℃, 1 h） could induce the expression of HSPs. Chemotaxis assay was used to observe the effect of TNF-a and heat shock response on RAW264.7 macrophages migration. It was found that TNF-α can induce the expression of inflammatory factors such as IL-1β、IL-6、IL-15; Heat shock response can induce the expression of HSP70, HSP90, HSP25; The migration of macrophages increased when treated with TNF-α, but heat shock response inhibited such effect. The result showed that heat shock response could inhibit RAW264.7 maerophages migration induced by TNF-α.

关 键 词：热休克预处理 TNF-Α 巨噬细胞 迁移 

分 类 号：R364.5[医药卫生—病理学]